Phosphorylated YDXV motifs and Nck SH2/SH3 adaptors act cooperatively to induce actin reorganization.

Molecular and Cellular Biology
Ivan M BlasutigT Pawson

Abstract

We have analyzed the means by which the Nck family of adaptor proteins couples adhesion proteins to actin reorganization. The nephrin adhesion protein is essential for the formation of actin-based foot processes in glomerular podocytes. The clustering of nephrin induces its tyrosine phosphorylation, Nck recruitment, and sustained localized actin polymerization. Any one of three phosphorylated (p)YDXV motifs on nephrin is sufficient to recruit Nck through its Src homology 2 (SH2) domain and induce localized actin polymerization at these clusters. Similarly, Nck SH3 mutants in which only the second or third SH3 domain is functional can mediate nephrin-induced actin polymerization. However, combining such nephrin and Nck mutants attenuates actin polymerization at nephrin-Nck clusters. We propose that the multiple Nck SH2-binding motifs on nephrin and the multiple SH3 domains of Nck act cooperatively to recruit the high local concentration of effectors at sites of nephrin activation that is required to initiate and maintain actin polymerization in vivo. We also find that YDXV motifs in the Tir protein of enteropathogenic Escherichia coli and nephrin are functionally interchangeable, indicating that Tir reorganizes the actin cytoske...Continue Reading

References

Sep 29, 1995·The Journal of Biological Chemistry·S BagrodiaR A Cerione
Oct 1, 1995·Molecular and Cellular Biology·O M Rivero-LezcanoK C Robbins
Mar 12, 1993·Cell·Z SongyangR J Lechleider
Dec 1, 1995·Molecular and Cellular Biology·M TanakaB J Mayer
Feb 6, 1996·Proceedings of the National Academy of Sciences of the United States of America·P van der GeerT Pawson
Oct 18, 1996·The Journal of Biological Chemistry·G M BokochU G Knaus
Aug 8, 1998·The Journal of Biological Chemistry·I M AntónR S Geha
Apr 17, 1999·Cellular Signalling·L WunderlichL Buday
Jul 8, 1999·Proceedings of the National Academy of Sciences of the United States of America·V RuotsalainenK Tryggvason
Dec 7, 2000·The American Journal of Pathology·V RuotsalainenH Jalanko
Oct 5, 2001·Nature Cell Biology·N K HussainP S McPherson
May 15, 2002·International Journal of Medical Microbiology : IJMM·Michelle L ZaharikB Brett Finlay
May 30, 2002·Cellular Signalling·László BudayPeter Tamás
Apr 2, 2003·The Journal of Biological Chemistry·Rakesh VermaLawrence B Holzman
Jul 9, 2003·Kidney International·Juhani LahdenperäKarl Tryggvason
Feb 6, 2004·The Journal of Cell Biology·Kenneth G CampelloneJohn M Leong
Dec 8, 2004·Journal of the American Society of Nephrology : JASN·Hongping LiTomoko Takano
Apr 26, 2006·The Journal of Biological Chemistry·Susanne FreseDirk W Heinz
Aug 29, 2006·Biochemical and Biophysical Research Communications·Hongping LiTomoko Takano
Sep 14, 2006·Proceedings of the National Academy of Sciences of the United States of America·Ivo QuackLorenz Sellin
Oct 10, 2007·Molecular and Cellular Biology·Puneet GargLawrence B Holzman

❮ Previous
Next ❯

Citations

Oct 26, 2011·Nature Reviews. Nephrology·Gavin I Welsh, Moin A Saleem
May 18, 2010·The Journal of Biological Chemistry·Puneet GargLawrence B Holzman
Nov 29, 2012·The Journal of Biological Chemistry·Laura A NewNina Jones
Jan 14, 2010·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Ying ZhangTadashi Yamamoto
Apr 2, 2011·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Fiona WuAshley M Toye
Jun 11, 2010·Annual Review of Biochemistry·Shae B Padrick, Michael K Rosen
Sep 2, 2011·American Journal of Physiology. Cell Physiology·Timur A AzhibekovJeffrey S Simske
Nov 9, 2012·Cell Communication and Signaling : CCS·Tomonori KanekoShawn Sc Li
May 8, 2009·Cell Communication and Signaling : CCS·Elvira Nieto-Pelegrin, Narcisa Martinez-Quiles
Jun 6, 2009·Journal of the American Society of Nephrology : JASN·Sagair HussainAnia Koziell
May 16, 2009·Journal of the American Society of Nephrology : JASN·Nina JonesTony Pawson
Jun 26, 2010·Journal of the American Society of Nephrology : JASN·Fiona WuAshley M Toye
Jul 31, 2012·International Journal of Nephrology·Juliana Reis MachadoMarlene Antônia Reis
May 8, 2008·FEBS Letters·Francisco M Vega, Anne J Ridley
Jan 24, 2016·Journal of the American Society of Nephrology : JASN·Laura A NewNina Jones
Mar 17, 2012·Experimental Cell Research·Puneet Garg, Lawrence B Holzman
Mar 1, 2016·Chemical Reviews·Veronika CsizmokJulie D Forman-Kay
Dec 2, 2009·The Journal of Pathology·Gavin I Welsh, Moin A Saleem
Jan 27, 2015·Journal of Diabetes Investigation·Yoshiro MaezawaKoutaro Yokote
Mar 30, 2011·Developmental Biology·Ahmed M Mohamed, Ian D Chin-Sang
Mar 24, 2012·Current Biology : CB·Joe Swift, Dennis E Discher
Sep 18, 2012·Cell Host & Microbe·Ashley C HumphriesMichael Way
Aug 31, 2016·Nature Reviews. Nephrology·Luca PericoGiuseppe Remuzzi
Oct 27, 2017·Proteomics·April L DarlingVladimir N Uversky
May 16, 2015·Journal of Leukocyte Biology·Dustin M E LillicoJames L Stafford
Oct 12, 2017·Journal of the American Society of Nephrology : JASN·Claire E MartinNina Jones
Feb 27, 2009·Journal of the American Society of Nephrology : JASN·Bernhard Schermer, Thomas Benzing
Jun 6, 2009·Journal of the American Society of Nephrology : JASN·Thomas Benzing
May 29, 2014·Current Opinion in Nephrology and Hypertension·Laura A NewNina Jones
Oct 11, 2019·Molecular Biology of the Cell·Soyeon KimMichael K Rosen

❮ Previous
Next ❯

Related Concepts

Related Feeds

Autoimmune Diseases

Autoimmune diseases occur as a result of an attack by the immune system on the body’s own tissues resulting in damage and dysfunction. There are different types of autoimmune diseases, in which there is a complex and unknown interaction between genetics and the environment. Discover the latest research on autoimmune diseases here.