Phosphorylation and activation of smooth muscle myosin light chain kinase by MAP kinase and cyclin-dependent kinase-1

Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire
D L MorrisonS L Pelech

Abstract

Smooth muscle myosin light chain kinase (MLCK) features several consensus sites of phosphorylation by proline-directed protein serine/threonine kinases. The phosphorylation of MLCK by two proline-directed kinases isolated from sea star oocytes, i.e., p44mpk (Mpk, a mitogen-activated protein kinase homologue) and cyclin-dependent kinase-1 (CDK1, also known as p34cdc2), was investigated. Chicken gizzard MLCK was phosphorylated on seryl and threonyl residues by both Mpk and CDK1. Phosphorylation of MLCK to 0.6 mol Pi/mol by Mpk increased the Vmax of phosphotransferase activity towards a synthetic peptide corresponding to residues 11-23 of the 20-kDa light chain of myosin by 1.6-fold. Phosphorylation of MLCK to 1.0 mol Pi/mol by CDK1 increased the Vmax by 2.3-fold. Phosphorylation by either kinase had no significant effect on the concentration of calmodulin required for half-maximal activation of MLCK. Analysis of the phosphorylation of synthetic peptides containing consensus phosphorylation sites for Mpk and CDK1 indicated that the major site of phosphorylation in MLCK by Mpk was Ser-834, and by CDK1 was Thr-283. Both of these sites are located outside the calmodulin-binding site (residues 796-815), consistent with the observation...Continue Reading

References

Dec 1, 1991·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·M P Walsh
May 18, 1990·Cell·S Moreno, P Nurse
Mar 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·N J OlsonA R Means
Mar 15, 1984·The Biochemical Journal·P K NgaiM P Walsh
Jan 1, 1983·Methods in Enzymology·M P WalshD J Hartshorne
May 1, 1995·Neurobiology of Aging·E MandelkowE M Mandelkow

❮ Previous
Next ❯

Citations

Nov 1, 1993·Molecular and Cellular Biochemistry·S L PelechJ S Sanghera
Jul 4, 2001·Thrombosis Research·A McNicolS J Israels
Sep 14, 2010·Human Reproduction Update·Stéphane Brunet, Marie Hélène Verlhac
Nov 7, 1998·American Journal of Respiratory Cell and Molecular Biology·A D VerinJ G Garcia
Mar 22, 2013·PloS One·Mykhaylo ArtamonovAvril V Somlyo
Apr 26, 2003·Archives of Physiology and Biochemistry·W J PearceW T Gerthoffer
Feb 2, 1999·The Journal of Physiology·M S KolodneyH F Yee
Sep 19, 2015·Cytoskeleton·Manuel Oropesa ÁvilaJosé Antonio Sánchez-Alcázar
Dec 14, 2004·Life Sciences·Ming-Ho Tsai, Meei Jyh Jiang
Nov 20, 2012·Cell Host & Microbe·Charlotte OdendallDavid W Holden
Dec 7, 2007·Biochemical and Biophysical Research Communications·Akio NakamuraKazuhiro Kohama
Jul 23, 2004·Physiological Reviews·Karl-Erik Andersson, Anders Arner
Apr 10, 2004·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Yunping LiKathleen G Morgan
Jun 28, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Joe G N GarciaAlexander D Verin
Nov 30, 2000·The Journal of Biological Chemistry·K E Kamm, J T Stull
Oct 24, 2002·The Journal of Biological Chemistry·Marnie S RobertsJim C Norman
Jun 19, 2001·American Journal of Physiology. Heart and Circulatory Physiology·P H Ratz
Jun 6, 2003·American Journal of Physiology. Lung Cellular and Molecular Physiology·Talaibek BorbievJoe G N Garcia
Jun 11, 1999·The American Journal of Physiology·J G GarciaV Natarajan
Jan 1, 2014·Journal of Smooth Muscle Research = Nihon Heikatsukin Gakkai Kikanshi·Charles D AndersonJohn R Grider
Mar 27, 2007·American Journal of Physiology. Heart and Circulatory Physiology·Renan J SandovalWilliam J Pearce
Apr 23, 2008·The Journal of Biological Chemistry·Sandrine HormanMark H Rider
Jun 16, 2001·Journal of Applied Physiology·G Pfitzer
Jan 15, 2002·American Journal of Physiology. Heart and Circulatory Physiology·Gerard D'Angelo, Leonard P Adam
Jul 29, 2021·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Dane W SorensenWilliam J Pearce
Dec 26, 2001·Chemical Reviews·T R Soderling, J T Stull

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Checkpoints & Regulators

Cell cycle checkpoints are a series of complex checkpoint mechanisms that detect DNA abnormalities and ensure that DNA replication and repair are complete before cell division. They are primarily regulated by cyclins, cyclin-dependent kinases, and the anaphase-promoting complex/cyclosome. Here is the latest research.