Phosphorylation and Interaction with the 14-3-3 Protein of the Plasma Membrane H+-ATPase are Involved in the Regulation of Magnesium-Mediated Increases in Aluminum-Induced Citrate Exudation in Broad Bean (Vicia faba. L)

Plant & Cell Physiology
Qi ChenLi-Mei Chen

Abstract

Several studies have shown that external application of micromolar magnesium (Mg) can increase the resistance of legumes to aluminum (Al) stress by enhancing Al-induced citrate exudation. However, the exact mechanism underlying this regulation remains unknown. In this study, the physiological and molecular mechanisms by which Mg enhances Al-induced citrate exudation to alleviate Al toxicity were investigated in broad bean. Micromolar concentrations of Mg that alleviated Al toxicity paralleled the stimulation of Al-induced citrate exudation and increased the activity of the plasma membrane (PM) H(+)-ATPase. Northern blot analysis shows that a putative MATE-like gene (multidrug and toxic compound extrusion) was induced after treatment with Al for 4, 8 and 12 h, whereas the mRNA abundance of the MATE-like gene showed no significant difference between Al plus Mg and Al-only treatments during the entire treatment period. Real-time reverse transcription-PCR (RT-PCR) and Western blot analyses suggest that the transcription and translation of the PM H(+)-ATPase were induced by Al but not by Mg. In contrast, immunoprecipitation suggests that Mg enhanced the phosphorylation levels of VHA2 and its interaction with the vf14-3-3b protein un...Continue Reading

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Citations

Nov 21, 2015·Molecular Plant·Janus FalhofMichael Palmgren
Sep 21, 2020·Physiologia Plantarum·Debojyoti KarSourav Datta
Jun 24, 2017·Frontiers in Plant Science·Ana P RibeiroHugo B C Molinari
Nov 2, 2017·Frontiers in Plant Science·Jiarong ZhangQi Chen
Oct 2, 2019·International Journal of Molecular Sciences·Yan-Mei WuLin-Tong Yang
Oct 10, 2018·International Journal of Molecular Sciences·Md Atikur RahmanKi-Won Lee
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Dec 4, 2020·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·Yang-Fei ZhouLin-Tong Yang
Nov 14, 2021·Plant Cell Reports·Ye HuangLiang Chen

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