Phosphorylation and metabolism of sucrose and its five linkage-isomeric alpha-D-glucosyl-D-fructoses by Klebsiella pneumoniae

Carbohydrate Research
J ThompsonFrieder W Lichtenthaler

Abstract

Not only sucrose but the five isomeric alpha-D-glucosyl-D-fructoses trehalulose, turanose, maltulose, leucrose, and palatinose are utilized by Klebsiella pneumoniae as energy sources for growth, thereby undergoing phosphorylation by a phosphoenolpyruvate-dependent phosphotransferase system uniformly at 0-6 of the glucosyl moiety. Similarly, maltose, isomaltose, and maltitol, when exposed to these conditions, are phosphorylated regiospecifically at O-6 of their non-reducing glucose portion. The structures of these novel compounds have been established unequivocally by enzymatic analysis, acid hydrolysis, FAB negative-ion spectrometry, and 1H and 13C NMR spectroscopy. In cells of K. pneumoniae, hydrolysis of sucrose 6-phosphate is catalyzed by sucrose 6-phosphate hydrolase from Family 32 of the glycosylhydrolase superfamily. The five 6'-O-phosphorylated alpha-D-glucosyl-fructoses are hydrolyzed by an inducible (approximately 49-50 Kda) phospho-alpha-glucosidase from Family 4 of the glycosylhydrolase superfamily.

References

Nov 1, 1979·Infection and Immunity·E J St Martin, C L Wittenberger
Sep 1, 1992·Infection and Immunity·Y Y Chen, D J LeBlanc
Jan 1, 1990·Annual Review of Biochemistry·N D MeadowS Roseman
Jan 1, 1990·Caries Research·T OoshimaS Sobue
Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·A FouetG Rapoport
Jul 1, 1982·Journal of Bacteriology·K SchmidR Schmitt
Jan 1, 1982·Methods in Enzymology·B M Chassy, E V Porter
Mar 1, 1994·Journal of Dental Research·J van Houte
Sep 1, 1993·Microbiological Reviews·P W PostmaG R Jacobson
Jun 1, 1993·Infection and Immunity·Y Y ChenD J LeBlanc
Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
May 22, 1998·Journal of Dentistry·P J Moynihan
Mar 21, 1953·Nature·H E WADE, D M MORGAN

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Citations

Jan 11, 2012·Molecular Oral Microbiology·J Thompson, A Pikis
Feb 10, 2017·Environmental Microbiology·Camille BlinSylvain Brisse
Dec 7, 2017·Nutrition Research and Practice·Joo-Yeon ChungYuri Kim
Apr 30, 2017·Applied and Environmental Microbiology·Philippe JoyetNicolas Sauvageot
Mar 8, 2002·Microbiology·Andreas PikisJohn Thompson
Jul 6, 2012·Natural Product Reports·David DaudéIsabelle André
Apr 25, 2020·Chemistry : a European Journal·Lifei LianYu-Fei Song
Mar 22, 2018·Frontiers in Microbiology·Bart J F KeijserRoy C Montijn
Aug 12, 2010·The Journal of Physical Chemistry. B·Wenjuan HuangJames W Gauld

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