PMID: 2109045May 1, 1990Paper

Phosphorylation by protein kinase C of the muscarinic acetylcholine receptor

Journal of Neurochemistry
K HagaA Ichiyama

Abstract

Muscarinic acetylcholine receptors purified from porcine cerebrum were phosphorylated by protein kinase C purified from the same tissue. More than 1 mol of phosphate was incorporated per mole of receptor, with both serine and threonine residues being phosphorylated. Neither the degree nor the rate of the phosphorylation was affected by the presence or absence of acetylcholine. GTP-sensitive high-affinity binding with acetylcholine was observed for muscarinic receptors reconstituted with GTP-binding proteins (Gi or Go), irrespective of whether muscarinic receptors or the GTP-binding proteins had been phosphorylated by protein kinase C or not. This indicates that the interaction between purified muscarinic receptors and purified GTP-binding proteins in vitro is not affected by their phosphorylation.

References

Mar 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·U WildenH Kühn
Feb 13, 1987·Biochemical and Biophysical Research Communications·A K HoJ H Wang
Jan 1, 1986·Methods in Enzymology·T KitanoY Nishizuka
Jan 1, 1983·Methods in Enzymology·J A CooperT Hunter

❮ Previous
Next ❯

Citations

Apr 1, 1993·Brain Research. Molecular Brain Research·E A Van der ZeeP G Luiten
May 3, 2003·Pharmacology & Therapeutics·Chris J van Koppen, Björn Kaiser
Jun 1, 1993·British Journal of Pharmacology·D R Bristow, M R Zamani
Jul 21, 1998·Annals of the New York Academy of Sciences·E Friedman, H Y Wang
Jun 14, 2013·Proceedings of the Japan Academy. Series B, Physical and Biological Sciences·Tatsuya Haga
Mar 31, 2009·International Journal for Parasitology·Michael J KimberPaula Ribeiro
Jun 1, 1998·Annals of the New York Academy of Sciences·Eitan Friedman, Hoau-Yan Wang

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.