Phosphorylation by protein kinase CK2 modulates the activity of the ATP binding cassette A1 transporter.

The Journal of Biological Chemistry
Stein RoosbeekM Rosseneu

Abstract

In a previous characterization of the ABCA subfamily of the ATP-binding cassette (ABC) transporters, we identified potential protein kinase 2 (CK2) phosphorylation sites, which are conserved in eukaryotic and prokaryotic members of the ABCA transporters. These phosphorylation residues are located in the conserved cytoplamic R1 and R2 domains, downstream of the nucleotide binding domains NBD1 and NBD2. To study the possible regulation of the ABCA1 transporter by CK2, we expressed the recombinant cytoplasmic domains of ABCA1, NBD1+R1 and NBD2+R2. We demonstrated that in vitro ABCA1 NBD1+R1, and not NBD2+R2, is phosphorylated by CK2, and we identified Thr-1242, Thr-1243, and Ser-1255 as the phosphorylated residues in the R1 domain by mass spectrometry. We further investigated the functional significance of the threonine and serine phosphorylation sites in NBD1 by site-directed mutagenesis of the entire ABCA1 followed by transfection into Hek-293 Tet-Off cells. The ABCA1 flippase activity, apolipoprotein AI and AII binding, and cellular phospholipid and cholesterol efflux were enhanced by mutations preventing CK2 phosphorylation of the threonine and serine residues. This was confirmed by the effect of specific protein kinase CK2 in...Continue Reading

References

Jan 1, 1992·Annual Review of Cell Biology·C F Higgins
Sep 26, 1997·Nature·M C Winter, M J Welsh
Feb 10, 1999·The American Journal of Physiology·C G VanoyeG A Altenberg
Jun 8, 1999·The Journal of Biological Chemistry·H H ChengR D Simoni
Sep 6, 2000·American Journal of Human Genetics·R Allikmets
Jan 21, 2001·The Journal of Biological Chemistry·O ChambenoitG Chimini
Mar 27, 2001·Current Opinion in Lipidology·G Schmitz, T Langmann
Apr 5, 2002·Current Atherosclerosis Reports·Gerd Schmitz, Wolfgang E Kaminski
Aug 23, 2002·Pharmacology & Therapeutics·Stefania SarnoLorenzo A Pinna
Aug 28, 2002·The Journal of Biological Chemistry·Raymond H SeeMichael R Hayden
Sep 26, 2002·FEBS Letters·Susan J MillerBenjamin G Neel
Nov 28, 2002·Journal of Lipid Research·Véronique RigotGiovanna Chimini
Dec 18, 2002·Annals of the New York Academy of Sciences·Véronique MartelClaude Cochet
Mar 13, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Flavio Meggio, Lorenzo A Pinna
Jul 19, 2003·The Journal of Biological Chemistry·Laurent O MartinezAlan R Tall
Mar 25, 2004·Protein Expression and Purification·Stein RoosbeekMaryvonne Rosseneu
Jun 10, 2004·European Journal of Mass Spectrometry·Angela AmoresanoEric Quemeneur

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Citations

Dec 6, 2005·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Emmanuel Boadu, Gordon A Francis
Feb 28, 2006·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Vassilis I ZannisMonty Krieger
Apr 26, 2008·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Nishi N Singh, Dipak P Ramji
Jul 22, 2006·Pflügers Archiv : European journal of physiology·Ana ZarubicaGiovanna Chimini
Jul 8, 2008·Molecular and Cellular Biochemistry·Anil Mehta
Jun 1, 2005·Biochimica Et Biophysica Acta·Gerd Schmitz, Thomas Langmann
Jul 5, 2011·Biochemistry·Yaroslav TsybovskyKrzysztof Palczewski
Feb 22, 2008·Journal of Cardiovascular Pharmacology·Jonas Heilskov GraversenSøren Kragh Moestrup
Sep 5, 2008·Current Opinion in Lipidology·Yves L MarcelMing-Dong Wang
Dec 27, 2005·FEBS Letters·Esther Biemans-OldehinkelBert Poolman
Sep 17, 2011·Biochimica Et Biophysica Acta·Yuhua Liu, Chongren Tang
Jul 13, 2013·IUBMB Life·Winnie LuuAndrew J Brown
May 11, 2011·The FEBS Journal·Kohjiro NagaoKazumitsu Ueda
Jun 27, 2006·Biochimica Et Biophysica Acta·Clara CavelierArnold von Eckardstein
Feb 24, 2006·Biochimica Et Biophysica Acta·Gregory BrubakerJonathan D Smith
Dec 6, 2011·The Biochemical Journal·Itziar Pinilla-MacuaMarçal Pastor-Anglada
Nov 17, 2012·Biochimica Et Biophysica Acta·Kohjiro NagaoKazumitsu Ueda
Sep 1, 2016·Small GTPases·Dmitri Sviridov, Nigora Mukhamedova
Jan 7, 2018·Journal of Lipid Research·Michael C Phillips
Oct 21, 2016·Arteriosclerosis, Thrombosis, and Vascular Biology·Nigora MukhamedovaDmitri Sviridov
Feb 28, 2006·The Journal of Biological Chemistry·Kei TakahashiKazumitsu Ueda
Feb 23, 2010·American Journal of Physiology. Endocrinology and Metabolism·Francesca IettaLuana Paulesu
May 22, 2019·Journal of Biochemistry·Taro WatanabeMichinori Matsuo
Oct 5, 2007·The Journal of Biological Chemistry·Douangsone D VadysirisackSissy M Jhiang
Sep 27, 2019·Pharmacological Reviews·Dongdong WangAtanas G Atanasov
Jan 20, 2021·The Journal of Steroid Biochemistry and Molecular Biology·Bhoomika SharmaNavneet Agnihotri

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