Phosphorylation by protein kinases A and C of myofibrillar proteins in rabbit stunned and non-stunned myocardium

Journal of Molecular and Cellular Cardiology
M MatejovicovaW Flameng

Abstract

We tested the hypothesis that altered phosphorylation of myofibrillar proteins is involved in post-ischemic myocardial stunning. Myofibrillar proteins were isolated from Langendorff perfused control rabbit hearts, hearts submitted to 15 min normothermic ischemia and hearts submitted to 15 min ischemia followed by 10 min of reperfusion (stunned hearts). The in vivo level of phosphorylation of specific contractile proteins by protein kinases A and C was indirectly detected by the amount of 32P incorporated in vitro in the presence of these protein kinases and saturating concentration of [gamma-32P]-ATP (back-phosphorylation method). In control experiments the back-phosphorylation technique was able to detect PKA- or PKC-induced protein phosphorylation in hearts treated with isoproterenol and phorbol ester, respectively. In stunned hearts, contractile function was significantly suppressed compared to the period before ischemia. We found no difference in myofibrillar protein profile (on densitometry of the Coomassie-stained gels after SDS-PAGE) and in PKA mediated 32P incorporation when comparing control, ischemic and stunned myocardium. Three different PKCs were used for phosphorylation: commercial purified rat brain PKC, partiall...Continue Reading

Citations

Aug 9, 2001·Coronary Artery Disease·J E Van Eyk, A M Murphy
Apr 3, 2007·Molecular Diagnosis & Therapy·Melanie Y White, Jennifer E Van Eyk
May 7, 2002·Journal of Molecular and Cellular Cardiology·John M Canty, Te-Chung Lee
Feb 24, 2009·Canadian Journal of Physiology and Pharmacology·Srilekha MaddikaNaranjan S Dhalla
Apr 16, 2014·Journal of Molecular and Cellular Cardiology·Margaret V Westfall
Sep 4, 1999·Circulation Research·D B Foster, J E Van Eyk
May 30, 2003·American Journal of Physiology. Cell Physiology·Erno Zádor, Frank Wuytack
May 18, 2001·American Journal of Physiology. Heart and Circulatory Physiology·H KöglerE Marbán
May 26, 2017·Molecular Cancer Research : MCR·Sukumar SarkarJames M Larner

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