PMID: 590264Nov 15, 1977

Phosphorylation from inorganic phosphate and ATP synthesis of sarcoplasmic membranes

European Journal of Biochemistry
F U BeilW Hasselbach

Abstract

The incorporation of inorganic phosphate in the fragmented sarcoplasmic membranes induced by the removal of calcium ions bound to high affinity binding sites at the cytoplasmic surface of the membranes gives rise to the formation of two species of phosphoenzyme. The properties of the phosphoproteins formed depend on the absence or the presence of a gradient of calcium ions across the membranes. The phosphoenzymes differ by the affinity of the protein for phosphate, the enthalpy of formation, the kinetics of phosphate incorporation, and by the sensitivity to ionophores and ADP. In the absence of a calcium gradient less than 0.5 nmol phosphoenzyme per mg protein are formed in media containing less than 5 mM phosphate at pH7 and 10 degrees C. Under the same conditions approximately 2 nmol of phosphoenzyme per mg protein are formed with an initial rate of 0.5 nmol mg-1-s-1 when a calcium gradient exists. When the gradient is abolished by the addition of the ionophore X537A, the level of phosphoprotein drops to the same value as observed in the absence of a gradient. On addition of ADP at concentrations increasing from 0.3 to 10 muM continuous ATP formation is activated to its maximum rate, and simultaneously, the level of phosphopr...Continue Reading

References

Jan 1, 1977·Annual Review of Biochemistry·E Racker
Dec 30, 1975·Annals of the New York Academy of Sciences·E RackerE Eytan
Nov 30, 1976·Biochemistry·Giuseppe InesiC R Coan
Aug 1, 1970·The Journal of General Physiology·P F Duggan, A Martonosi
Dec 1, 1972·The Journal of General Physiology·A ScarpaGiuseppe Inesi
May 28, 1971·European Journal of Biochemistry·W Fiehn, A Migala
Jan 30, 1971·FEBS Letters·M Makinose, W Hasselbach
May 20, 1960·Biochimica Et Biophysica Acta·M AVRON
Apr 3, 1962·Biochemical and Biophysical Research Communications·W Hasselbach, M Makinose

Citations

Jan 1, 1982·Annals of the New York Academy of Sciences·L de Meis
Nov 14, 1978·Biochemistry·S Verjovski-AlmeidaGiuseppe Inesi
Feb 15, 2002·The Journal of Biological Chemistry·Carlota SumbillaGiuseppe Inesi

Related Concepts

Magnesium ADP
Adenosine Triphosphate, Chromium Ammonium Salt
Calcium
Mathematics
Tissue Membrane
Orthophosphate
Phosphoproteins
Sarcoplasmic Reticulum
Thermodynamics

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