PMID: 2503376Aug 1, 1989Paper

Phosphorylation in vitro of vimentin by protein kinases A and C is restricted to the head domain. Identification of the phosphoserine sites and their influence on filament formation

European Journal of Biochemistry
N GeislerK Weber

Abstract

The in vitro phosphorylation of vimentin, the intermediate filament protein of mesenchymal cells, by kinases A and C is serine-specific and involves only the N-terminal head domain. In oligomeric protofilament units each kinase recognizes five sites, which have been identified by sequence analysis. Kinase C introduces 1.5 mol phosphate/mol vimentin, while kinase A treatment results in 4 mol phosphate/mol. Kinase-A-treated oligomers do not polymerize in standard assays whereas kinase C treatment has no inhibitory effect. Filaments exposed to kinase A remain stable and incorporate only 1.7 mol phosphate/mol vimentin. These phosphates are essentially restricted to two of the five kinase A sites found in protofilament units. Thus the head domain, previously related to in vitro assembly competence and filament stability, changes in accessibility between the oligomeric and polymeric state. We discuss the possibility that in vivo phosphorylation of vimentin filaments by kinase A may not necessarily be accompanied by an extensive depolymerization. It could instead involve a dynamic change of the filament surfaces, which could alter the interaction of the filaments with other cellular structures.

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Citations

Jan 1, 1996·Cell Motility and the Cytoskeleton·B S Eckert, P L Yeagle
Aug 27, 2013·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Praveena S ThiagarajanMartha K Cathcart
Dec 1, 1993·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·J Ciesielski-TreskaD Aunis
Nov 1, 1990·The Journal of Cell Biology·J M RaatsH Bloemendal
Nov 1, 1992·The Journal of Cell Biology·J A DentM W Klymkowsky
Nov 1, 1995·The Journal of Cell Biology·M OgawaraH Sugiura
Feb 17, 2009·Biochemistry. Biokhimii︠a︡·A A Minin, M V Moldaver
Oct 23, 2003·Annual Review of Cell and Developmental Biology·Brian T HelfandRobert D Goldman
May 1, 1992·Journal of Neurochemistry·A S BenderM D Norenberg
Feb 1, 1992·Current Opinion in Cell Biology·J E ErikssonR D Goldman
Oct 13, 2004·Journal of Structural Biology·Harald BärHarald Herrmann
Mar 29, 1991·Biochemical and Biophysical Research Communications·S AndoM Inagaki
Mar 30, 2001·The Journal of Biological Chemistry·C M SahlgrenJ E Eriksson
Mar 29, 2002·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Masaaki TakemuraShigeyoshi Itohara
Jun 24, 2016·Journal of Cell Science·Maxfield P FlynnMary Hunzicker-Dunn
Sep 1, 1994·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·H SalmhoferH Denk
Feb 18, 2005·Toxicological Sciences : an Official Journal of the Society of Toxicology·M B MorettoJ B T Rocha
Jan 1, 1991·Cell Motility and the Cytoskeleton·O Skalli, R D Goldman

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