PMID: 9426286Feb 28, 1998Paper

Phosphorylation modulates L-type Ca channels in frog ventricular myocytes by changes in sensitivity to Mg2+ block

Pflügers Archiv : European journal of physiology
K Yamaoka, I Seyama

Abstract

The effect of phosphorylation on the intracellular Mg2+ concentration-dependent change in Ca channel activity was examined using the patch clamp technique. The kinetic changes in Ca channels induced either by phosphorylation [1 muM forskolin (FSK) plus 50 muM isobutylmethylxanthine (IBMX)] or by lowering intracellular [Mg2+] ([Mg2+]i) are qualitatively identical: an increase in both the open probability and availability of channels, as well as a decrease in the closed time without a change in the mean open time. This suggests that the mechanism for the increase in activity of Ca channels shares a common pathway of kinetic change. The concentration/response curve for the Mg2+-evoked modification of calcium channels was altered greatly by channel phosphorylation. In the external medium containing 1 muM FSK + 50 muM IBMX, Ca channels recorded with pipettes containing okadaic acid (OA) lost their sensitivity to Mg2+ in the range 1 x 10(-6) M-1 x 10(-3) M and remained in a fully active state. On the contrary, under basal conditions, the activity of Ca channel was strongly dependent on the internal Mg2+ over the same range of [Mg2+]. Similarly, phosphorylation of Ca channels eliminated the blocking action of guanosine triphosphate ob...Continue Reading

Citations

Jan 23, 2003·Molecular Aspects of Medicine·Pietro Delva
Dec 20, 2012·The Journal of General Physiology·Sylvain BrunetWilliam A Catterall
Feb 1, 2002·Biochemical and Biophysical Research Communications·Giuliano MeyerMarkus Paulmichl
Nov 18, 2003·The Journal of Physiology·Min WangJoshua R Berlin
Feb 17, 2006·American Journal of Physiology. Cell Physiology·Min Wang, Joshua R Berlin
Feb 9, 2002·American Journal of Physiology. Heart and Circulatory Physiology·Kaoru YamaokaIssei Seyama
Sep 15, 2001·American Journal of Physiology. Heart and Circulatory Physiology·S PelzerD J Pelzer
Nov 23, 2006·Molecular and Cellular Biochemistry·Sajal ChakrabortiTapati Chakraborti
Nov 13, 2015·American Journal of Physiology. Cell Physiology·Jianjun XuMasaki Kameyama
Feb 24, 2001·Critical Care Clinics·M S Agus, Z S Agus
Nov 25, 2006·Archives of Biochemistry and Biophysics·Kanigula MubagwaRegina Macianskiene

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