PMID: 8457194Mar 15, 1993Paper

Phosphorylation of adenosine in anoxic hepatocytes by an exchange reaction catalysed by adenosine kinase

The Biochemical Journal
F BontempsG Van den Berghe

Abstract

The elevation of adenosine levels induced by anoxia in isolated rat hepatocytes has been shown to result mainly from an arrest of the recycling of the nucleoside by adenosine kinase [Bontemps, Vincent and Van den Berghe (1993) Biochem. J. 290, 671-677]. To assess the activity of the latter enzyme in intact hepatocytes, incorporation of radioactive adenosine into the cells' adenine nucleotides was measured. Unexpectedly, despite the near-absence of ATP in anoxic cells, 40% of 50 microM [8-14C]adenosine was still incorporated into adenylates over 5 min. Moreover, whereas unlabelled and labelled adenosine were utilized in parallel in normoxic cells, uptake of [8-14C]adenosine did not correspond to a net disappearance of adenosine in anoxic cells. Addition of 1 mM unlabelled adenosine to anoxic hepatocytes in which the adenine nucleotides had been prelabelled with [U-14C]adenine induced an immediate loss of their radioactivity. The latter was recovered in the form of adenosine, but the size of the adenylate pool was not modified. Taken together, these results suggest the occurrence of an exchange reaction between AMP and adenosine. Incubation of Sephadex G-25-filtered high-speed supernatants of rat liver with 20 microM [8-14C]adeno...Continue Reading

Citations

Dec 4, 2008·The FEBS Journal·Daniela VannoniEnrico Marinello
Feb 7, 2013·Neurochemistry International·Eleonora AronicaDetlev Boison
Dec 29, 1998·Biological Chemistry·A L KovácsP O Seglen
Aug 12, 2018·Scientific Reports·Renata Rocha de OliveiraKleber Gomes Franchini
Apr 18, 2013·Pharmacological Reviews·Detlev Boison
Oct 18, 2008·The Journal of Biological Chemistry·Maria Veiga-da-CunhaEmile Van Schaftingen
May 9, 2002·Proceedings of the National Academy of Sciences of the United States of America·Detlev BoisonHanns Mohler
Jan 1, 1995·Pharmacology & Therapeutics·E S Arnér, S Eriksson

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