PMID: 697756Jul 15, 1978Paper

Phosphorylation of adenosine monophosphate in the mitochondrial matrix

The Biochemical Journal
H A Krebs, D Wiggins

Abstract

The origin of the GTP needed for th phosphorylation of AMP in the mitochondrial matrix was investigated. When short-chain fatty acids are metabolized by hepatocytes, AMP is readily formed within the matrix by the butyryl-CoA ligase (AMP-forming) reaction (EC 6.2.1.2). The rate of matrix AMP formation in rat hepatocytes was calculated from the rate of ketone-body formation. The rate of the reconversion of matrix AMP into ADP by GTP-AMP transphosphorylase is limited by the rate of supply of GTP. GTP can be formed either by succinic thiokinase (EC 6.2.1.4) or by nucleoside diphosphokinase (EC 2.7.4.6). The rate of the succinic thiokinase reaction was calculated from turnover of the tricarboxylic acid cycle and this was calculated from the rate of O2 consumption and ketone-body formation. The results show that nucleoside diphosphokinase can make a major contribution (up to 80%) to the supply of GTP under the test conditions.

Citations

May 5, 2009·Biochimica Et Biophysica Acta·Uwe SchlattnerRichard M Epand
Oct 15, 1984·European Journal of Biochemistry·P HuitorelD Pantaloni
Oct 27, 2009·BMC Evolutionary Biology·Thomas DesvignesJulien Bobe
Nov 2, 1993·Biochimica Et Biophysica Acta·L Wojtczak, P Schönfeld
Mar 2, 2018·Laboratory Investigation; a Journal of Technical Methods and Pathology·Marie-Lise LacombeUwe Schlattner
Apr 24, 2009·Molecular and Cellular Biochemistry·Mathieu BoissanMarie-Lise Lacombe
May 9, 2000·The Journal of Biological Chemistry·L MilonM L Lacombe
Apr 3, 2020·Immunological Reviews·Peter J McGuire
Mar 1, 1995·Molecular and Biochemical Parasitology·R M UlloaM T Tellez-Iñón
Dec 7, 1988·Biochimica Et Biophysica Acta·P SchönfeldL Wojtczak

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