Phosphorylation of an N-terminal motif enhances DNA-binding activity of the human SRY protein.

The Journal of Biological Chemistry
M DesclozeauxP Berta

Abstract

Of the several strategies that eukaryotes have evolved to modulate transcription factor activity, phosphorylation is regarded as one of the major mechanisms in signal-dependent transcriptional control. To conclusively demonstrate that the human sex-determining gene SRY is affected by such a post-translational control mechanism, we have analyzed its phosphorylation status in living cells. In the present study, we show that the cyclic AMP-dependent protein kinase (PKA) phosphorylates the human SRY protein in vitro as well as in vivo on serine residues located in the N-terminal part of the protein. This phosphorylation event was shown to positively regulate SRY DNA-binding activity and to enhance the ability of SRY to inhibit a basal promoter activity located downstream of an SRY DNA-binding site concatamer. Together these results strongly support the hypothesis that human SRY is a natural substrate for PKA in vivo and that this phosphorylation significantly modulates its major activity, DNA-binding, thereby possibly altering its biological function.

References

Aug 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·U M MollA J Levine
Aug 7, 1992·Cell·T Hunter, M Karin
May 9, 1991·Nature·P KoopmanR Lovell-Badge
Sep 24, 1990·Biochimica Et Biophysica Acta·L A Pinna
Jan 1, 1983·Methods in Enzymology·J D DignamR G Roeder
Mar 1, 1995·The Journal of Cell Biology·F PoulatP Berta
Dec 1, 1993·Human Molecular Genetics·C ClépetP N Goodfellow
May 10, 1994·Proceedings of the National Academy of Sciences of the United States of America·D R CohenJ D McGovern
Apr 25, 1994·Nucleic Acids Research·V R HarleyP N Goodfellow
Dec 1, 1993·Genes & Development·S Natesan, M Z Gilman
Aug 19, 1993·Nature·L S WhitfieldP N Goodfellow
Apr 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·K McElreaveyM Fellous
May 25, 1993·Nucleic Acids Research·V LaudetH Clevers
Mar 15, 1996·Nucleic Acids Research·M Lnenicek-AllenC Crane-Robinson
Aug 5, 1996·FEBS Letters·V R HarleyP J Hextall
Jan 1, 1995·Reproduction, Fertility, and Development·P Koopman
Jan 1, 1996·Current Topics in Developmental Biology·A Greenfield, P Koopman
Jun 1, 1997·Current Opinion in Genetics & Development·L H Pevny, R Lovell-Badge

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Dec 26, 2001·Archives of Medical Research·H Merchant-Larios, N Moreno-Mendoza
Jun 23, 2001·Molecular and Cellular Endocrinology·R HabertJ M Saez
Nov 1, 2002·Molecular Genetics and Metabolism·Claire L Mitchell, Vincent R Harley
Dec 21, 2012·Journal of Proteome Research·Zohreh JangraviGhasem Hosseini Salekdeh
Sep 5, 2013·Proceedings of the National Academy of Sciences of the United States of America·Yen-Shan ChenMichael A Weiss
Aug 1, 2013·Proceedings of the National Academy of Sciences of the United States of America·Yen-Shan ChenMichael A Weiss
May 6, 2008·Molecular Human Reproduction·I Sánchez-MorenoP Canto
Dec 22, 1999·Nucleic Acids Research·T TchénioT Heidmann
Feb 13, 2009·Biology of Reproduction·Stephen T BradfordDagmar Wilhelm
Oct 26, 2011·Sexual Development : Genetics, Molecular Biology, Evolution, Endocrinology, Embryology, and Pathology of Sex Determination and Differentiation·P Parma, O Radi
Nov 16, 2013·BMC Genomics·Jeremy W ProkopAmy Milsted
Aug 2, 2007·Molecular Endocrinology·Leo DiNapoli, Blanche Capel
May 24, 2003·Proceedings of the National Academy of Sciences of the United States of America·Vincent R HarleyDavid A Jans
Dec 19, 2001·The Journal of Experimental Zoology·L Salas-CortésM Rosemblatt
Nov 17, 2009·Steroids·Daniel ElyAmy Milsted
Jul 5, 2008·The International Journal of Biochemistry & Cell Biology·Pascal BernardVincent Harley
Jul 1, 2008·Trends in Endocrinology and Metabolism : TEM·Helena SimVincent R Harley
Aug 7, 2004·The EMBO Journal·Laurie ThevenetBrigitte Boizet-Bonhoure
Jul 1, 2008·Biology of the Cell·Juan Carlos PolancoPeter Koopman
Sep 6, 2015·European Journal of Cell Biology·Zhen-Yu She, Wan-Xi Yang
Mar 1, 2000·Experimental Cell Research·W M KulykL M Hoffman
Sep 26, 2006·Developmental Biology·Juan Carlos Polanco, Peter Koopman
Aug 16, 2016·Seminars in Cell & Developmental Biology·Linlin HouRalf Jauch
Jan 24, 2007·Physiological Reviews·Dagmar WilhelmPeter Koopman
May 12, 2009·Cell Biology International·Youichi SatoYutaka Nakahori
Mar 18, 2006·Arquivos brasileiros de endocrinologia e metabologia·Maricilda Palandi de MelloChristine Hackel
Jul 28, 2005·Journal of Biochemistry·Yoshiakira KanaiTomohide Kidokoro
Nov 11, 2010·Development·Kenichi Kashimada, Peter Koopman
Dec 26, 2001·Chemical Reviews·J B Shabb

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