Abstract
Phosphorylation is a major post-translational modification of αB-crystallin (CryaB) and determines this protein's chaperone activity, intracellular distribution, translocation, and cytoprotective functions. Phosphorylation of CryaB manifests itself as either beneficial or deleterious consequences depending on the extent of phosphorylation and interaction with the cytoskeleton. Herein, for the first time, we compared the age-related alterations of the expression and phosphorylation (on Ser59: pS59) of CryaB in the myocardium of Wistar and senescence-accelerated OXYS rats. The latters, as we demonstrated here, develop cardiomyopathy by the age of 12 months against the background of hypertension. Rats at the age of 20 days, 3, 12, and 24 months were used. The expression of CryaB mRNA (studied by RT-PCR) and of the CryaB protein (analyzed by western blotting) increased with age in the myocardium of both Wistar and OXYS rats, but only at the age of 24 months did their levels become lower in OXYS rats. Phosphorylation of CryaB increased with age in all rats. There was no association of cardiomyopathy with the pS59-CryaB amount in the detergent-soluble fraction either. Moreover, immunostaining of the myocardium revealed that the amoun...Continue Reading
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