Abstract
Transcription by nonsegmented negative-strand RNA viruses is mediated by the viral RNA-dependent RNA polymerase and transcriptional cofactor P. The P protein is activated by phosphorylation, an event initiated by cellular kinases. The kinase used differs among this group of RNA viruses; vesicular stomatitis virus and respiratory syncytial virus utilize casein kinase II (CKII), whereas human parainfluenza virus type 3 utilizes PKC isoform zeta (PKC-zeta) for activation of its P protein. To identify the cellular kinase(s) involved in the phosphorylation of the canine distemper virus (CDV) P protein, we used recombinant CDV P in phosphorylation assays with native kinase activities present in CV1 cell extracts or purified CKII and PKC isoforms. Here, we demonstrate that the CDV P protein is phosphorylated by two cellular kinases, where PKC-zeta has the major and CKII the minor activities. In contrast, the P protein of another member of the morbillivirus genus, measles virus, is phosphorylated predominantly by CKII, whereas PKC-zeta has only minor activity. Selective inhibition of PKC-zeta activity within CV1 cells eliminated permissiveness to CDV replication, indicating an in vivo role for PKC-zeta in the virus replication cycle. T...Continue Reading
References
Jul 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·S Barik, A K Banerjee
Aug 1, 1992·Virology·J CurranD Kolakofsky
Oct 23, 1992·Science·Y Nishizuka
Sep 1, 1992·Molecular and Cellular Biology·I DominguezJ Moscat
Sep 1, 1992·Virology·M YamanakaT Yilma
Jan 1, 1991·Pharmacology & Therapeutics·A K BanerjeeB P De
Apr 1, 1991·Journal of Virology·S Barik, A K Banerjee
Jun 1, 1991·Journal of Virology·B P DeA K Banerjee
Sep 1, 1991·The Journal of Cell Biology·I J YuD R Marshak
Jun 15, 1990·Cell·P LarroderaJ Moscat
Jun 1, 1989·Journal of Virological Methods·M OglesbeeS Krakowka
Sep 1, 1989·The Journal of General Virology·M OglesbeeS Krakowka
Aug 25, 1988·Nature·Y Nishizuka
Jun 1, 1983·The Journal of General Virology·B K Rima
Jul 15, 1983·Virology·M HamaguchiY Nagai
Apr 1, 1981·Journal of Virology·M C Graves
Jan 30, 1980·Virology·W W HallP W Choppin
Nov 10, 1995·Virology·S BarikL C Dupuy
Oct 13, 1995·The Journal of Biological Chemistry·T DasA K Banerjee
Aug 1, 1995·Virology·T DasA K Banerjee
Aug 20, 1995·Virology·C C HuntleyA K Banerjee
Aug 13, 1993·Cell·E BerraJ Moscat
Apr 28, 1995·The Journal of Biological Chemistry·J A GoodnightJ F Mushinski
Apr 20, 1995·Virology·A M Takacs, A K Banerjee
May 23, 1995·Proceedings of the National Academy of Sciences of the United States of America·B P DeA K Banerjee
Feb 1, 1995·The Journal of General Virology·A K GuptaA K Banerjee
Nov 15, 1994·Virology·B Mazumder, S Barik
Mar 1, 1994·The Journal of General Virology·N VillanuevaI García-Albert
Aug 1, 1993·Molecular and Cellular Biology·M T Diaz-MecoJ Moscat
Sep 1, 1993·European Journal of Biochemistry·G KochsT F Sarre
Citations
Dec 10, 1999·Journal of Virology·A K GuptaA K Banerjee
Dec 22, 2009·Future Microbiology·Sandra M FuentesBiao He
Oct 2, 2012·FEBS Letters·Akihiro SugaiChieko Kai
Apr 30, 2008·Proteomics·Kyoji HagiwaraChieko Kai
Jun 15, 1999·Virology·C A TidonaG Darai
Nov 2, 1999·Virology·A KohlM Bouloy
Feb 1, 2008·Virology·Judith M FontanaPaul A Rota
Apr 10, 1999·Electrophoresis·B Guerra, O G Issinger
Nov 27, 2015·Journal of Virology·Adrian PickarBiao He
Feb 7, 2014·Journal of Virology·Adrian PickarBiao He
Oct 2, 2008·Journal of Medical Entomology·Chelsea T Smartt, Jennifer S Erickson
Nov 9, 2000·Molecular Biology and Evolution·T B StibitzD Bhattacharya
Mar 13, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Flavio Meggio, Lorenzo A Pinna
Dec 10, 1999·The Journal of Biological Chemistry·T OginoK Mizumoto
Dec 6, 2000·The Journal of Biological Chemistry·H VihinenL Kääriäinen
Mar 3, 2004·The Journal of General Virology·Rajnish Kaushik, M S Shaila
Jun 14, 2000·The Journal of Biological Chemistry·H Vihinen, J Saarinen
Sep 29, 1999·Pharmacology & Therapeutics·J Lenard
Jul 13, 2004·Virus Research·Tamal RahaM S Shaila