Phosphorylation of canine distemper virus P protein by protein kinase C-zeta and casein kinase II

Virology
Z LiuM J Oglesbee

Abstract

Transcription by nonsegmented negative-strand RNA viruses is mediated by the viral RNA-dependent RNA polymerase and transcriptional cofactor P. The P protein is activated by phosphorylation, an event initiated by cellular kinases. The kinase used differs among this group of RNA viruses; vesicular stomatitis virus and respiratory syncytial virus utilize casein kinase II (CKII), whereas human parainfluenza virus type 3 utilizes PKC isoform zeta (PKC-zeta) for activation of its P protein. To identify the cellular kinase(s) involved in the phosphorylation of the canine distemper virus (CDV) P protein, we used recombinant CDV P in phosphorylation assays with native kinase activities present in CV1 cell extracts or purified CKII and PKC isoforms. Here, we demonstrate that the CDV P protein is phosphorylated by two cellular kinases, where PKC-zeta has the major and CKII the minor activities. In contrast, the P protein of another member of the morbillivirus genus, measles virus, is phosphorylated predominantly by CKII, whereas PKC-zeta has only minor activity. Selective inhibition of PKC-zeta activity within CV1 cells eliminated permissiveness to CDV replication, indicating an in vivo role for PKC-zeta in the virus replication cycle. T...Continue Reading

References

Jul 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·S Barik, A K Banerjee
Sep 1, 1992·Molecular and Cellular Biology·I DominguezJ Moscat
Jan 1, 1991·Pharmacology & Therapeutics·A K BanerjeeB P De
Sep 1, 1991·The Journal of Cell Biology·I J YuD R Marshak
Sep 1, 1989·The Journal of General Virology·M OglesbeeS Krakowka
Jun 1, 1983·The Journal of General Virology·B K Rima
May 23, 1995·Proceedings of the National Academy of Sciences of the United States of America·B P DeA K Banerjee
Aug 1, 1993·Molecular and Cellular Biology·M T Diaz-MecoJ Moscat
Sep 1, 1993·European Journal of Biochemistry·G KochsT F Sarre

❮ Previous
Next ❯

Citations

Dec 22, 2009·Future Microbiology·Sandra M FuentesBiao He
Nov 9, 2000·Molecular Biology and Evolution·T B StibitzD Bhattacharya
Mar 13, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Flavio Meggio, Lorenzo A Pinna
Dec 10, 1999·The Journal of Biological Chemistry·T OginoK Mizumoto
Dec 6, 2000·The Journal of Biological Chemistry·H VihinenL Kääriäinen
Jun 14, 2000·The Journal of Biological Chemistry·H Vihinen, J Saarinen

❮ Previous
Next ❯

Related Concepts