Phosphorylation of C/EBPbeta at a consensus extracellular signal-regulated kinase/glycogen synthase kinase 3 site is required for the induction of adiponectin gene expression during the differentiation of mouse fibroblasts into adipocytes

Molecular and Cellular Biology
Bae-Hang ParkS R Farmer

Abstract

Stimulation of adipogenesis in mouse preadipocytes requires C/EBPbeta as well as activation of the MEK/extracellular signal-regulated kinase (ERK) signaling pathway. In this study, we demonstrate that phosphorylation of C/EBPbeta at a consensus ERK/glycogen synthase kinase 3 (GSK3) site regulates adiponectin gene expression during the C/EBPbeta-facilitated differentiation of mouse fibroblasts into adipocytes. First, we show that exposure of 3T3-L1 preadipocytes to insulin, dexamethasone (DEX), and isobutylmethylxanthine (MIX) leads to the phosphorylation of C/EBPbeta at threonine 188. Pretreating the cells with a MEK1-specific inhibitor (U0126) significantly attenuates this activity. Similarly, these effectors activate the phosphorylation of T188 within an ectopic C/EBPbeta overexpressed in Swiss mouse fibroblasts, and this event involves both MEK1 and GSK3 activity. We further show that expression of C/EBPbeta (p34kD LAP isoform) in Swiss mouse fibroblasts exposed to DEX, MIX, and insulin induces expression of peroxisome proliferator-activated receptor gamma (PPARgamma) and some adiponectin but that it does not activate expression of FABP4/aP2. In fact, complete conversion of these fibroblasts into lipid-laden adipocytes, whic...Continue Reading

References

Mar 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·T NakajimaS Akira
Nov 7, 1998·The Journal of Biological Chemistry·G J DarlingtonO A MacDougald
Dec 22, 1999·Current Opinion in Cell Biology·Z WuB M Spiegelman
Jan 5, 2000·Molecular Cell·E Kowenz-Leutz, A Leutz
Jun 8, 2000·Annals of the New York Academy of Sciences·J K HammS R Farmer
Sep 27, 2001·Journal of Cellular Physiology·E M EatonL Sealy
Oct 19, 2001·Proceedings of the National Academy of Sciences of the United States of America·H G LinhartG J Darlington
Jan 10, 2002·Genes & Development·Evan D RosenBruce M Spiegelman
Dec 28, 2002·Proceedings of the National Academy of Sciences of the United States of America·Qi-Qun TangM Daniel Lane
Feb 28, 2003·The Journal of Biological Chemistry·Teddy T C Yang, Chi-Wing Chow
Jul 2, 2003·Molecular and Cellular Biology·Michelle N BradleyStephen T Smale

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Citations

May 1, 2008·Molecular Biology Reports·Liangzhi ZhangHanyang Zhang
Jan 25, 2012·Cell Biochemistry and Biophysics·Jin-Gai ZhuXi-Rong Guo
Jun 7, 2005·Molecular and Cellular Neurosciences·Sylvain NadeauFreda D Miller
Sep 29, 2011·Nature Reviews. Molecular Cell Biology·Ana G Cristancho, Mitchell A Lazar
Oct 21, 2009·The Journal of Biological Chemistry·Buer SenJanet Rubin
Apr 15, 2008·The Journal of Biological Chemistry·Hyuk C ChaOrmond A MacDougald
Feb 27, 2009·The Journal of Biological Chemistry·Isabelle GerinOrmond A Macdougald
Jul 25, 2007·Molecular and Cellular Biology·Olivier BezyC Ronald Kahn
Feb 6, 2008·Molecular and Cellular Biology·Padmaja GadeDhananjaya V Kalvakolanu
Aug 19, 2005·Molecular and Cellular Biology·Robert L KortumRobert E Lewis
Feb 18, 2011·American Journal of Physiology. Endocrinology and Metabolism·Liping QiaoJianhua Shao
Dec 17, 2009·American Journal of Physiology. Gastrointestinal and Liver Physiology·Zheng ShenMin You
Feb 5, 2011·Molecular Endocrinology·Tracy X CuiJessica Schwartz
Mar 10, 2009·PloS One·Jean-Philippe CoutureRichard Blouin
Feb 16, 2011·Journal of Molecular Endocrinology·Raghunath ChatterjeeCharles Vinson
Oct 4, 2012·The Journal of Endocrinology·M L BatistaM Seelaender
Nov 11, 2011·International Journal of Molecular Sciences·Pablo CalzadillaLiliana N Guerra
Mar 25, 2014·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·C CaselliD Giannessi
Jul 2, 2014·The Journal of Clinical Investigation·Marcelo A MoriC Ronald Kahn
Aug 5, 2009·Critical Reviews in Clinical Laboratory Sciences·Elzbieta Gocek, George P Studzinski
Jun 3, 2009·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Normand PodechardValérie Lecureur
Oct 2, 2004·Obesity Reviews : an Official Journal of the International Association for the Study of Obesity·S R Farmer, J Auwerx
Oct 24, 2007·Alcoholism, Clinical and Experimental Research·James J Potter, Esteban Mezey
Sep 13, 2016·Adipocyte·Erica L SchellerWilliam P Cawthorn
Feb 5, 2009·Proceedings of the National Academy of Sciences of the United States of America·Vincent MarionHélène Dollfus
Feb 13, 2016·Toxicological Sciences : an Official Journal of the Society of Toxicology·Alicia M BoltKoren K Mann
Oct 10, 2015·Adipocyte·Carla Cervantes-CamachoWalid Kuri-Harcuch
Dec 17, 2009·The Biochemical Journal·Meilian Liu, Feng Liu
Nov 13, 2014·Cell Biochemistry and Biophysics·Susmita Bag, Anand Anbarasu
Aug 15, 2013·The International Journal of Biochemistry & Cell Biology·Enrico RagniLorenza Lazzari
Dec 22, 2014·Lipids in Health and Disease·Andressa Bolsoni-LopesMaria Isabel C Alonso-Vale
Jan 31, 2006·Diabetes·Myriam AouadiFrédéric Bost

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