Phosphorylation of CRMP2 is required for migration and positioning of Purkinje cells: Redundant roles of CRMP1 and CRMP4.

Brain Research
Yuki YamazakiToshio Ohshima

Abstract

Proper migration and positioning of Purkinje cells are important for formation of the developing cerebellum. Although several cyclin-dependent kinase 5 (Cdk5) substrates are known to be critical for ordered neuronal migration, there are no reports of mutant mouse-based, in vivo studies on the function of Cdk5-phosphorylation substrates in migration of Purkinje cells. We focused on the analysis of collapsin response mediator protein 2 (CRMP2), one of the Cdk5 substrates, because a previous study reported migration defects of cortical neurons with shRNA-mediated knockdown of CRMP2. However, CRMP2 KI/KI mice, in which Cdk5-phosphorylation is inhibited, showed little defects in Purkinje cell migration and positioning. We hypothesized compensatory redundant functions of the other CRMPs, and analyzed the migration and positioning of Purkinje cells in the cerebellum in every combination of CRMP1 knockout (KO), CRMP2 KI/KI, and CRMP4 KO mice. Severe disturbance of migration and positioning of Purkinje cells were observed in the triple mutant mice. We also found motor coordination defects in the triple CRMPs mutant mice. These results suggest the importance of both, phosphorylation of CRMP2 by Cdk5 and the redundant functions of CRMP1 a...Continue Reading

References

Nov 10, 1995·The Journal of Biological Chemistry·D TangJ H Wang
Oct 1, 1996·Proceedings of the National Academy of Sciences of the United States of America·T OhshimaA B Kulkarni
Dec 31, 1997·Journal of Neurochemistry·L H Wang, S M Strittmatter
Nov 14, 2002·Molecular Neurobiology·Toshio Ohshima, Katsuhiko Mikoshiba
Jan 18, 2005·Cell·Takeshi YoshimuraKozo Kaibuchi
Sep 27, 2005·Experimental Cell Research·Volker RosslenbroichJoachim Kappler
Nov 24, 2006·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Emmanuelle CharrierPappachan Kolattukudy
Dec 22, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Naoya YamashitaYoshio Goshima
Nov 3, 2010·Neuropsychology Review·Joan Stiles, Terry L Jernigan
Jan 12, 2012·Developmental Neurobiology·Emi NiisatoToshio Ohshima
Jan 27, 2012·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Naoya YamashitaYoshio Goshima
Oct 23, 2012·Molecular and Cellular Neurosciences·A KumazawaT Ohshima
Sep 13, 2013·Frontiers in Neuroanatomy·M Albert Basson, Richard J Wingate
Mar 4, 2014·PloS One·Jerry SkefosMargaret Bauman
Sep 17, 2014·The Journal of Biological Chemistry·Mohamad R KhazaeiAlyson E Fournier
Jan 30, 2015·Frontiers in Neuroscience·Toshio Ohshima
Nov 26, 2015·The Journal of Biological Chemistry·Hui-Chia Yu-Kemp, William M Brieher
Jan 31, 2018·Molecular Neurodegeneration·Jifeng GuoShihua Li

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Citations

Aug 30, 2020·Molecular Neurobiology·Sabine C WinklerJosef P Kapfhammer
May 25, 2021·Frontiers in Molecular Neuroscience·Camille CuveillierAnnie Andrieux

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