Phosphorylation of E47 as a potential determinant of B-cell-specific activity.

Molecular and Cellular Biology
Steven R SloanT Kadesch

Abstract

The E2A gene encodes two basic helix-loop-helix proteins designated E12 and E47. Although these proteins are widely expressed, they are required only for the B-lymphocyte lineage where DNA binding is mediated distinctively by E47 homodimers. By studying the properties of deltaE47, an N-terminal truncation of E47, we provide evidence that phosphorylation may contribute to B-cell-specific DNA binding by E47. Two serines N terminal to the deltaE47 basic helix-loop-helix domain were found to be phosphorylated in a variety of cell types but were hypophosphorylated in B cells. Phosphorylating these serines in vitro inhibited DNA binding by deltaE47 homodimers but not by deltaE47-containing heterodimers, such as deltaE47:MyoD. These results argue that hypophosphorylation may be a prerequisite for activity of E47 homodimers in B cells, suggesting the use of an inductive (nonstochastic) step in early B-cell development.

References

Oct 11, 1992·Molecular Biology of the Cell·S Y ChouJ E Ferrell
Jan 1, 1992·Molecular and Cellular Biology·J M PongubalaM L Atchison
Jan 1, 1991·Genes & Development·D RuezinskyT Kadesch
Feb 1, 1991·Molecular and Cellular Biology·C MurreD Baltimore
Apr 1, 1973·Virology·F L Graham, A J van der Eb
May 1, 1967·Developmental Biology·J L Curry, J J Trentin
Apr 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·T SudaM Ogawa
Sep 1, 1982·Molecular and Cellular Biology·C M GormanB H Howard
Aug 1, 1995·Molecular and Cellular Biology·C P Shen, T Kadesch
Jun 21, 1994·Biochimica Et Biophysica Acta·C MurreM H Stuiver
Dec 31, 1993·Cell·M A RudnickiR Jaenisch
Jun 1, 1993·Molecular and Cellular Biology·G BainC Murre
Jan 1, 1964·Proceedings of the National Academy of Sciences of the United States of America·J E TILLL SIMINOVITCH

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Citations

Nov 17, 2010·Developmental and Comparative Immunology·Jun-ichi HikimaTakashi Aoki
Sep 18, 2007·Molecular Immunology·Jun-ichi HikimaGregory W Warr
May 27, 2010·Journal of Cellular Biochemistry·Jun-Ho LeeCynthia M Smas
Jan 25, 2006·Seminars in Immunology·Stephanie Roessler, Rudolf Grosschedl
Sep 1, 2005·Biochemical and Biophysical Research Communications·Robyn J Kewley, Murray L Whitelaw
Jun 20, 2001·Experimental Cell Research·J R BeckerS E Johnson
Feb 12, 2013·Cell Reports·Owen R DaviesSally Lowell
Apr 5, 2013·PloS One·Eva CubilloAmparo Cano
May 23, 1998·Annual Review of Immunology·A Henderson, K Calame
Mar 23, 2004·Annual Review of Immunology·Meinrad Busslinger
Nov 12, 2015·Developmental Cell·Lan-Hsin Wang, Nicholas E Baker
Aug 26, 1999·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·E V RothenbergM K Anderson
Jan 6, 2009·Proceedings of the National Academy of Sciences of the United States of America·Melissa A ThalChristopher A Klug
May 31, 2012·Human Molecular Genetics·Lev PrasovTom Glaser
Feb 19, 2005·The EMBO Journal·Frederic LluísPura Muñoz-Cánoves
Jan 12, 2007·Bulletin of Mathematical Biology·Olivier Cinquin, Karen M Page
Oct 22, 2005·Nature Immunology·Cornelis Murre
Sep 25, 1999·The Journal of Biological Chemistry·G S HugginsM E Lee
Feb 23, 2012·Molecular and Cellular Biology·Robert TeachenorCornelis Murre
May 11, 2005·Immunological Reviews·Richard L RileyDaniela Frasca
Mar 22, 2002·Nature Reviews. Immunology·I Engel, C Murre
Nov 12, 2010·The Journal of Biological Chemistry·Guergana TassevaJean E Vance
Dec 15, 2000·The Journal of Biological Chemistry·C Chu, D S Kohtz
Feb 13, 2004·The Journal of Biological Chemistry·Robert MånssonMikael Sigvardsson
Apr 7, 1998·Molecular and Cellular Biology·P OrdentlichT Kadesch
Jan 11, 2002·Molecular and Cellular Biology·Sabine HerblotTrang Hoang

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