Phosphorylation of FtsZ and FtsA by a DNA Damage-Responsive Ser/Thr Protein Kinase Affects Their Functional Interactions in Deinococcus radiodurans

MSphere
Ganesh K MauryaHari S Misra

Abstract

Deinococcus radiodurans, a highly radioresistant bacterium, does not show LexA-dependent regulation of recA expression in response to DNA damage. On the other hand, phosphorylation of DNA repair proteins such as PprA and RecA by a DNA damage-responsive Ser/Thr protein kinase (STPK) (RqkA) could improve their DNA metabolic activities as well as their roles in the radioresistance of D. radiodurans Here we report RqkA-mediated phosphorylation of cell division proteins FtsZ and FtsA in vitro and in surrogate Escherichia coli bacteria expressing RqkA. Mass spectrometric analysis mapped serine 235 and serine 335 in FtsZ and threonine 272, serine 370, and serine 386 in FtsA as potential phosphorylation sites. Although the levels of FtsZ did not change during postirradiation recovery (PIR), phosphorylation of both FtsZ and FtsA showed a kinetic change during PIR. However, in an rqkA mutant of D. radiodurans, though FtsZ underwent phosphorylation, no kinetic change in phosphorylation was observed. Further, RqkA adversely affected FtsA interaction with FtsZ, and phosphorylated FtsZ showed higher GTPase activity than unphosphorylated FtsZ. These results suggest that both FtsZ and FtsA are phosphoproteins in D. radiodurans The increased ph...Continue Reading

References

Jan 1, 1991·Analytical Biochemistry·T P GeladopoulosA E Evangelopoulos
Apr 6, 1995·Biochemical and Biophysical Research Communications·A HigashitaniK Horiuchi
May 20, 1998·Proceedings of the National Academy of Sciences of the United States of America·G KarimovaD Ladant
Sep 21, 2002·Molecular Genetics and Genomics : MGG·C Bonacossa de AlmeidaA Bailone
Mar 9, 2005·Molecular Microbiology·Sebastien Pichoff, Joe Lutkenhaus
Nov 2, 2005·Nature Reviews. Microbiology·Michael M Cox, John R Battista
Mar 22, 2006·Nucleic Acids Research·Ivan MijakovicDusica Vujaklija
Sep 1, 2006·Molecular Microbiology·Ashwini ChauhanMalini Rajagopalan
Sep 29, 2006·Nature·Ksenija ZahradkaMiroslav Radman
Oct 28, 2006·The Journal of Biological Chemistry·Meghna Thakur, Pradip K Chakraborti
Mar 3, 2007·Annual Review of Biochemistry·Joe Lutkenhaus
Dec 19, 2007·Molecular Microbiology·Hidetaka OginoHideaki Yukawa
Aug 15, 2009·Nature Reviews. Microbiology·David W Adams, Jeff Errington
Feb 26, 2010·Cold Spring Harbor Perspectives in Biology·Martin Thanbichler
Nov 30, 2010·The EMBO Journal·Nam Ky TonthatMaria A Schumacher
Feb 16, 2011·Proceedings of the National Academy of Sciences of the United States of America·Hongbaek ChoThomas G Bernhardt
Mar 5, 2011·Microbiology and Molecular Biology Reviews : MMBR·Dea Slade, Miroslav Radman
Jan 1, 2007·Biochemistry and Molecular Biology Education : a Bimonthly Publication of the International Union of Biochemistry and Molecular Biology·Asta KausaiteArunas Ramanavicius
Nov 25, 2011·DNA Repair·Michael J Daly
Mar 21, 2012·Proceedings of the National Academy of Sciences of the United States of America·Katrin BeilharzJan-Willem Veening
Jul 31, 2012·Methods : a Companion to Methods in Enzymology·Aurélia Battesti, Emmanuelle Bouveret
Apr 10, 2013·Trends in Cell Biology·Hirofumi HarashimaArp Schnittger
Jul 16, 2013·Environmental Microbiology·Orietta MassiddaWaldemar Vollmer
Dec 10, 2013·Nature Cell Biology·Martin Loose, Timothy J Mitchison
Sep 18, 2015·FEMS Microbiology Reviews·Cristina OrtizMiguel Vicente
Oct 3, 2015·FEMS Microbiology Reviews·Sylvie ManuseChristophe Grangeasse
Jun 4, 2016·The Journal of Biological Chemistry·Yogendra S RajpurohitHari S Misra

❮ Previous
Next ❯

Citations

Jan 29, 2019·Journal of Biomolecular Structure & Dynamics·Dhirendra Kumar SharmaYogendra Singh Rajpurohit
Aug 14, 2019·Journal of Bacteriology·Lauren R HammondPrahathees J Eswara
Nov 27, 2019·International Journal of Molecular Sciences·Paula YagüeAngel Manteca
Feb 25, 2021·The Journal of Biological Chemistry·Ganesh K MauryaHari S Misra
May 8, 2021·Frontiers in Cell and Developmental Biology·Yogendra Singh RajpurohitHari S Misra

❮ Previous
Next ❯

Methods Mentioned

BETA
GTPase
immunoprecipitation
bacterial
coimmunoprecipitation
co-IP
PCR
surface plasmon resonance
chip
transgenic

Software Mentioned

GraphPad Prism5
AutoLab
Image J2
GraphPad Prism
AutoLab Esprit
OriginPro
ImageJ

Related Concepts

Related Feeds

Cell Cycle Pathways

Cell cycle is a complex process regulated by several signal transduction pathways and enzymes. Here is the latest research on regulation of cell cycle and cell cycle pathways.