Phosphorylation of IRS1 at serine 307 and serine 312 in response to insulin in human adipocytes

Biochemical and Biophysical Research Communications
Anna DanielssonPeter Strålfors

Abstract

Feedback control in insulin signaling involves serine phosphorylation of insulin receptor substrate-1 (IRS1). By analyzing the insulin-induced phosphorylation of IRS1 at serine 307, serine 312, and tyrosine in the same primary human adipocytes, we now report that negative feedback phosphorylation of serine 312 (corresponding to murine serine 307) required relatively high concentrations of insulin (EC(50)=3 nM) for a long time (t(1/2) ca. 30 min) and reduced the steady-state tyrosine phosphorylation, without affecting the cellular concentration, of IRS1. In contrast, positive feedback phosphorylation of serine 307 was a rapid (t(1/2) ca. 2 min) event at physiological concentrations of insulin (EC(50)=0.2 nM).

References

Nov 6, 2002·The Journal of Biological Chemistry·Yong Hee LeeMorris F White
Jan 3, 2003·The Journal of Biological Chemistry·Michael W GreeneRichard A Roth
Jan 15, 2004·Diabetologia·L PirolaE Van Obberghen
Jan 18, 2005·Trends in Biochemical Sciences·Laura S HarringtonRichard F Lamb
May 3, 2005·The Journal of Clinical Investigation·Kathryn E Wellen, Gökhan S Hotamisligil

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Citations

Jul 14, 2009·Molecular Medicine·Anna DanielssonPeter Strålfors
Aug 14, 2009·Archives of Physiology and Biochemistry·Sonia Fernández-VeledoMargarita Lorenzo
Apr 25, 2014·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Heather F PidcokeCharles E Wade
Jun 19, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Anita OstPeter Strålfors

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