PMID: 8947546Dec 1, 1996Paper

Phosphorylation of linker histone is associated with transcriptional activation in a normally silent nucleus

The Journal of Cell Biology
M T SweetC D Allis

Abstract

Previous studies have suggested that micronuclear linker histones are phosphorylated by cAMP-dependent protein kinase (PKA) in Tetrahymena (Sweet, M.T., and C.D. Allis. 1993. Chromosoma. 102: 637-647). In this study, we report that a rapid and dramatic phosphorylation of the micronuclear linker histone, delta, occurs early in the sexual pathway, conjugation. Phosphorylated isoforms of delta are detected as early as 30 min after mixing cells of different mating types; blocking pair formation abolishes this induction completely. Phosphorylation of delta is stimulated by the addition of N6-benzoyladenosine 3':5' cyclic monophosphate to starved (nonmating) cells, suggesting that a PKA/cAMP signal transduction pathway is involved. Maximal phosphorylation of delta is observed during meiotic prophase, a period when micronuclei become transcriptionally active. In situ staining, using phospho-delta-specific antibodies combined with [3H]uridine autoradiography, shows that decondensed micronuclear chromatin undergoing active transcription is enriched in phosphorylated delta isoforms. In contrast, condensed inactive micronuclear chromatin is enriched in dephosphorylated delta. These results strongly suggest that phosphorylation of linker h...Continue Reading

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Citations

Sep 15, 2011·The Journal of Biological Chemistry·Ursula E Schoeberl, Kazufumi Mochizuki
Jan 16, 1999·Nucleic Acids Research·A P Wolffe, J J Hayes
Nov 13, 1998·Proceedings of the National Academy of Sciences of the United States of America·H HuangC D Allis
Jul 27, 2002·Annual Review of Microbiology·Carolyn L Jahn, Lawrence A Klobutcher
Jul 27, 2001·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·G R Green
Sep 7, 2000·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·J ZlatanovaK Van Holde
Jan 10, 1997·The Journal of Biological Chemistry·M T SweetC D Allis

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