PMID: 11319150Apr 25, 2001Paper

Phosphorylation of mitogen-activated protein kinase is regulated by protein kinase C, cyclic 3',5'-adenosine monophosphate, and protein phosphatase modulators during meiosis resumption in rat oocytes

Biology of Reproduction
Qing LuQ Y Sun

Abstract

Mitogen-activated protein (MAP) kinase, protein kinase C (PKC), cAMP, and okadaic acid (OA)-sensitive protein phosphatases (PPs) have been suggested to be involved in oocyte meiotic resumption. However, whether these protein kinases and phosphatases act by independent pathways or interact with each other in regulating meiosis resumption is unknown. In the present study, we aimed to determine the regulation of meiosis resumption and MAP kinase phosphorylation by PKC, cAMP, and OA-sensitive PPs in rat oocytes using an in vitro oocyte maturation system and Western blot analysis. We found that ERK1 and ERK2 isoforms of MAP kinases existed in a dephosphorylated (inactive) form in germinal vesicle breakdown (GVBD)-incompetent and GVBD-competent germinal vesicle intact (GVI) oocytes as well as GVBD oocytes at equivalent levels. These results indicate that MAP kinases are not responsible for the initiation of normal meiotic resumption in rat oocytes. However, when GVBD-incompetent and GVBD-competent oocytes were incubated in vitro for 5 h, MAP kinases were phosphorylated (activated) in GVBD-competent oocytes, but not in meiotic-incompetent oocytes, suggesting that oocytes acquire the ability to phosphorylate MAP kinase during acquisiti...Continue Reading

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Citations

Oct 30, 2004·Molecular Reproduction and Development·T L Smythe, S A Stricker
Oct 1, 2010·Zygote : the Biology of Gametes and Early Embryos·G Sánchez ToranzoM I Bühler
Apr 16, 2004·Biology of Reproduction·Amy Inselman, Mary Ann Handel
Aug 22, 2012·Reproductive Biology and Endocrinology : RB&E·Xiao-Yu DuYue-Hui Zheng
Mar 25, 2008·Fertility and Sterility·Claudia Messias GomesGary D Smith
Jan 1, 2009·Molecular Reproduction and Development·Stephen A Stricker
Feb 14, 2008·Molecular Reproduction and Development·Shao-Chen SunQing-Yuan Sun
Jun 26, 2010·Molecular Reproduction and Development·Stephen M Downs
May 30, 2006·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Abha Mishra, Keerikkattil Paily Joy
Mar 1, 2005·Molecular Reproduction and Development·Xin TanYong-Zheng Tang
Feb 3, 2007·Zygote : the Biology of Gametes and Early Embryos·G Sánchez ToranzoM I Bühler
May 31, 2007·Molecular Endocrinology·Cheng-Guang LiangQing-Yuan Sun
Feb 8, 2019·Cell Cycle·Zi-Yun YiWei-Ping Qian
Mar 16, 2021·Frontiers in Cell and Developmental Biology·Wen-Long LeiQing-Yuan Sun
Dec 1, 2004·Molecular Reproduction and Development·Cean LaRosa, Stephen M Downs
Jun 22, 2021·Frontiers in Cell and Developmental Biology·Yu WuMo Yang

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