Phosphorylation of myelin basic protein in intact oligodendrocytes: inhibition by galactosylsphingosine and cyclic AMP.

Glia
T VartanianS Szuchet

Abstract

We have previously shown that cyclic AMP (cAMP) inhibits the protein kinase C (PKC)-mediated phosphorylation of myelin basic protein (MBP) in cultured oligodendrocytes (OLGs). Recently, it has been demonstrated that the long chain base sphingosine inhibits PKC by competing PKC effectors (diacylglycerol and phorbol esters) for a binding site on the kinase (Hannun and Bell: Science 235: 670-674, 1987). In this report we define further the mechanism by which cAMP inhibits MBP phosphorylation by comparing the effects of cAMP with that of galactosylsphingosine (psychosine), a potential catabolite of galactocerebroside, the major OLG glycosphingolipid. We identify the consequences of psychosine treatment and PKC down-regulation on OLG morphology and electrophysiology and discuss their relevance. Our results in intact ovine oligodendrocytes are consistent with a mechanism in which cAMP inhibits MBP phosphorylation by interfering with the release of diacylglycerol (DAG) from phosphatidylinositol. First, the effects of cAMP on MBP phosphorylation are reversed with exogenous TPA; and second, cAMP inhibits the incorporation of 1-[14C]arachidonate into DAG and specifically inhibits the turnover (as judged by 32PO4 3-incorporation) of phosp...Continue Reading

References

Feb 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·T VartanianS Szuchet
Jan 1, 1985·Annual Review of Biochemistry·L E Hokin
Oct 1, 1988·The Journal of Membrane Biology·B SolivenD J Nelson
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Aug 1, 1981·Pflügers Archiv : European journal of physiology·O P HamillF J Sigworth

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Citations

Mar 29, 2001·Microscopy Research and Technique·R L Stariha, S U Kim
Nov 1, 1991·The Journal of Membrane Biology·B SolivenD J Nelson
Jun 1, 1995·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·B Soliven, S Szuchet
Mar 1, 1997·Journal of Neuroimmunology·S M LeVine, D C Brown
Oct 16, 2015·Apoptosis : an International Journal on Programmed Cell Death·A C E GrazianoV Cardile
Sep 18, 2016·Journal of Neuroscience Research·Rosanna AvolaVenera Cardile
Sep 18, 2016·Journal of Neuroscience Research·Stefka Spassieva, Erhard Bieberich
Jan 29, 2021·The Journal of Biological Chemistry·Lisa G LippertSivaraj Sivaramakrishnan

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