PMID: 3754389Apr 1, 1986Paper

Phosphorylation of myosin in permeabilized mammalian cardiac and skeletal muscle cells

The American Journal of Physiology
H L Sweeney, J T Stull

Abstract

The effect of myosin phosphorylation on tension production at less than 50% maximal activation by Ca2+ was examined in rabbit psoas and ventricular muscle. For psoas fibers, tension was determined at pCa 6.0, 5.8, 5.6, 5.5, and 5.4. Myosin light chain kinase (0.15 microM) and calmodulin (2 microM) were added, and the fibers were incubated at pCa 5.4, which resulted in an increase in light chain phosphorylation (P-light chain) from 5-10 to 60-75%. After 5 min, the sequence of pCa activations was repeated. An identical protocol was followed for cardiac muscle, except the activation solutions were pCa 6.2, 6.0, 5.9, 5.8, and 5.6. Phosphorylation of P-light chain increased tension in both permeabilized cardiac and skeletal muscle fibers. The effect manifested itself as a leftward shift in the pCa-tension relationship at levels below 50% maximal activation, with a decrease in the slope of the pCa-tension relationship. These results indicate that P-light chain phosphorylation affects actin-myosin interactions in cardiac and skeletal muscles at submaximal levels of Ca2+ activation.

References

Sep 12, 1979·Biochemical and Biophysical Research Communications·D R Manning, J T Stull
Jan 1, 1985·Annual Review of Pharmacology and Toxicology·K E Kamm, J T Stull
Jan 1, 1986·Journal of Molecular and Cellular Cardiology·P J SilverJ T Stull

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Citations

Oct 1, 1989·Clinical Physiology·H J Green, S R Jones
May 13, 1998·Acta Physiologica Scandinavica·H J Green
Jun 14, 2000·Journal of Applied Physiology·B R MacIntosh, J C Willis
Apr 25, 2000·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·D E Rassier, B R Macintosh
Jun 11, 2002·American Journal of Physiology. Cell Physiology·F AbbateH Westerblad
Feb 12, 2005·Journal of Applied Physiology·Ali E Oskouei, Walter Herzog
Jan 16, 2008·Physiological Reviews·D G AllenH Westerblad
Dec 30, 2009·The Journal of Biological Chemistry·Audrey N ChangJames T Stull
Oct 26, 2010·Journal of Muscle Research and Cell Motility·Ian Curtis SmithRene Vandenboom
Oct 29, 2013·Journal of Muscle Research and Cell Motility·Rene VandenboomJames T Stull
Dec 14, 2018·The Journal of General Physiology·Jason J BreithauptBertrand C W Tanner
Apr 11, 2020·The Journal of Biological Chemistry·Arnab NayakMamta Amrute-Nayak
Feb 1, 1995·Journal of Muscle Research and Cell Motility·R W Kensler, J L Woodhead
May 29, 1999·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·D E RassierB R MacIntosh
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Jul 15, 2015·Bioarchitecture·Sarah M Heissler, James R Sellers

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