Phosphorylation of myosin light chain from adrenomedullary chromaffin cells in culture.

The Biochemical Journal
Luis M GutiérrezSalvador Viniegra

Abstract

The myosin-light-chain (MLC) phosphorylation accompanying catecholamine release in chromaffin cells was investigated with the objective of assessing the possible role of this contractile protein in catecholamine secretion. The electrophoretic characteristics of adrenomedullary MLC were determined by immunochemical techniques using two different specific antibodies. The identified 22 kDa phosphoprotein was mainly present in the cytosol, as demonstrated by ultracentrifugation and immunocytochemical analysis. A part of this protein was located on, or close to, the plasma membrane. Cell stimulation by secretagogues resulted in a Ca2(+)-dependent 32P incorporation into MLC, the time course of this process being related to catecholamine release. These findings were supported by a two-dimensional gel-electrophoretic analysis by which means this protein was resolved into two acidic forms. A role for Ca2(+)-calmodulin and Ca2(+)-phospholipid kinases in adrenomedullary MLC phosphorylation is reported. The results obtained suggest a regulatory role for such a protein in the underlying exocytotic event.

Citations

Nov 21, 2007·Acta Physiologica·J-M TrifaróL M Gutiérrez
Oct 13, 2006·Biochimica Et Biophysica Acta·Magali MalacombeStéphane Gasman
Apr 8, 2004·The Journal of Biological Chemistry·Patricia NecoLuis M Gutiérrez
Dec 11, 2007·Pflügers Archiv : European journal of physiology·Yumi Noda, Sei Sasaki
Sep 12, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Hang ShiMichael B Zemel
Oct 26, 2018·Frontiers in Cellular Neuroscience·Yolanda Gimenez-MolinaLuis M Gutiérrez
Jul 22, 2017·Pflügers Archiv : European journal of physiology·Luis M Gutiérrez, José Villanueva
May 20, 2017·Frontiers in Cellular Neuroscience·Yolanda Gimenez-MolinaLuis M Gutiérrez
Jun 25, 2005·Journal of Cell Science·Daniel GinerLuis M Gutiérrez
Jul 22, 1991·Biochimica Et Biophysica Acta·R D Burgoyne
Jun 30, 1992·Biochemical and Biophysical Research Communications·M Ohara-ImaizumiK Kumakura

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