Phosphorylation of neurofibromatosis type 1 gene product (neurofibromin) by cAMP-dependent protein kinase

FEBS Letters
I IzawaHideyuki Saya

Abstract

The critical function of the neurofibromatosis type 1 (NF1) gene product (neurofibromin) is not well defined except that neurofibromin has homology with a family of the GTPase-activating proteins (GAPs). In this study, we confirmed that neurofibromin is constitutively phosphorylated and detected kinase activities which specifically phosphorylate the cysteine/serine-rich domain and the C-terminal domain of the neurofibromin in cell lysate. In vitro and in-gel kinase assays strongly indicated that cAMP-dependent protein kinase (PKA) is a candidate for the neurofibromin kinase. THe biological significance of the phosphorylation of neurofibromin is unclear at present, but we speculate that neurofibromin plays a crucial role in cellular function since it links the two major cellular pathways which are the GAP-ras and PKA-associated signals.

Citations

Jun 8, 2002·Biochemical and Biophysical Research Communications·Dieter KaufmannBritta Bartelt
Jul 11, 1998·Biochimica Et Biophysica Acta·R Sanchez-OleaK Strange
Jan 11, 2000·Journal of Medical Genetics·J SzudekJ M Friedman
Jul 23, 2011·Genes & Cancer·Thomas GrewalCarlos Enrich
Feb 13, 2004·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Gladys Y-P KoStuart E Dryer
Mar 28, 2006·The Tohoku Journal of Experimental Medicine·Masahito HatoriShoichi Kokubun
Jan 15, 2014·Child's Nervous System : ChNS : Official Journal of the International Society for Pediatric Neurosurgery·Salma Ben-SalemLihadh Al-Gazali
May 13, 2014·Advances in Biological Regulation·Ophélia Maertens, Karen Cichowski
Mar 8, 2011·Neurochemical Research·Ian Dang, George H De Vries
Dec 30, 2008·Protein Expression and Purification·Fabien BonneauKlaus Scheffzek
Jul 4, 2006·Clinical Genetics·A B Trovó-Marqui, E H Tajara
Jun 14, 2013·Annals of Human Genetics·Martina NemethovaAndrea Zatkova
Jan 13, 2006·Biochemical and Biophysical Research Communications·Madanamohan BoyanapalliDaniel D Mikol
Nov 26, 2011·Bioscience Reports·Victoria A Barron, Hua Lou
Apr 5, 2011·The American Journal of Pathology·Eeva-Mari JouhilahtiJuha Peltonen
Feb 28, 2004·FEBS Letters·Ina VandenbrouckeLudwine Messiaen
Aug 15, 2018·Cold Spring Harbor Perspectives in Medicine·Klaus Scheffzek, Giridhar Shivalingaiah
Dec 15, 2019·The Journal of Biological Chemistry·Mukul SherekarDominic Esposito
Aug 26, 1998·The Canadian Journal of Neurological Sciences. Le Journal Canadien Des Sciences Neurologiques·M M FeldkampA Guha
Oct 31, 2020·Cells·Mohammed BergougHélène Bénédetti
Jun 3, 2005·Cancer Research·Lawrence S KirschnerConstantine A Stratakis
Oct 25, 2020·Cancers·Arianna Bellazzo, Licio Collavin
Dec 7, 2000·Journal of Neuroscience Research·M M Lakkis, G I Tennekoon
Jun 4, 2021·Molecular Genetics & Genomic Medicine·Nahla N Abdel-AzizKhalda S Amr

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