Phosphorylation of osteopontin peptides mediates adsorption to and incorporation into calcium oxalate crystals

Cells, Tissues, Organs
Jason O'YoungGraeme K Hunter

Abstract

Phosphorylated peptides of osteopontin (OPN) have been shown to inhibit the growth of the {100} face of calcium oxalate monohydrate (COM). The inhibitory potency has been shown to be dependent on the phosphate content of the peptide. The purpose of this study is to better understand the means by which phosphate groups promote crystal growth inhibition by OPN peptides. Peptides of rat bone OPN 220-235 peptides have been synthesized with zero (P0), 1 (P1) or 3 (P3) phosphate modifications. COM crystals were grown in the presence of 0.1-10 microg of P0, P1 or P3. P0 incorporation into COM crystals was evident at 10 microg/ml of peptide, whereas the phosphorylated peptides P1 and P3 were incorporated at all tested concentrations. At 5 microg/ml of P3, COM crystals exhibited a 'dumbbell' morphology. To study the peptide-mineral interaction, surface frequency plots were constructed from molecular dynamics simulations of OPN peptide adsorption. Carboxylate and phosphate groups were found to adsorb in specific orientations to the COM {100} surface. In conclusion, it appears that the phosphate groups on OPN peptides are capable of interacting with the COM {100} surface. This interaction appears to increase the adsorption energy of the p...Continue Reading

Citations

May 11, 2010·Circulation Journal : Official Journal of the Japanese Circulation Society·Ming-Song HsiehHorng-Mo Lee
Feb 23, 2010·PloS One·Paul V AzzopardiGraeme K Hunter
Jan 26, 2016·Matrix Biology : Journal of the International Society for Matrix Biology·Adele L Boskey, Eduardo Villarreal-Ramirez
Mar 26, 2011·Hepatology Research : the Official Journal of the Japan Society of Hepatology·Lin YangXi-Liang Zha
Dec 4, 2016·Urolithiasis·Jeffrey D RimerJeffrey A Wesson
Nov 23, 2017·Chemical Reviews·Alexander G ShtukenbergBart Kahr
Apr 25, 2013·Langmuir : the ACS Journal of Surfaces and Colloids·Shailesh S NeneJeffrey L Hutter
Dec 12, 2012·Crystal Growth & Design·Keith M BromleyJanet Moradian-Oldak

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