Phosphorylation of P42/P44 MAP kinase and DNA fragmentation in the rat perforant pathway stimulation model of limbic epilepsy

Brain Research
Jonathan L BrismanA J Cole

Abstract

The intracellular signaling pathways associated with neuronal injury after perforant pathway stimulation of the rodent hippocampus have not been examined. To determine whether activation of the p42/p44 (Erk1/2) MAP kinase (MAPK) phosphorylation cascade is linked to neuronal injury after perforant pathway stimulation (PPS), we stained for phosphorylated Erk1/2 (P-Erk1/2) and for DNA fragmentation, a marker of cell death after PPS. Eighteen Sprague-Dawley rats underwent PPS for 6 (n=6), 12 (n=6), or 24 (n=6) h and were sacrificed either immediately (n=9) or 48 h (n=9) after stimulation. Sham-operated non-stimulated control animals (n=2) and control animals receiving low frequency stimulation only (n=4) were also examined. Brain sections were stained for DNA fragmentation and P-Erk1/2. DNA fragmentation was evident only in granule cells and CA3 pyramidal cells of the stimulated side 48 h after 24 h of PPS. PPS resulted in robust phosphorylation of Erk1/2 that displayed a stereotyped timecourse, appearing first in hilar neurons on the ipsilateral side and later in hilar neurons, granule cells, hippocampal pyramidal and non-neuronal cell populations on both the stimulated and contralateral sides. Both Erk1/2 phosphorylation and DNA ...Continue Reading

References

Nov 24, 1995·Science·Z XiaM E Greenberg
Jan 1, 1993·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·J H WijsmanJ H van Dierendonck
Jul 21, 1994·Neuroreport·R K FilipkowskiL Kaczmarek
Aug 1, 1997·The Journal of Biological Chemistry·J D English, J D Sweatt
Sep 18, 1997·Proceedings of the National Academy of Sciences of the United States of America·J BengzonO Lindvall
May 29, 1998·Brain Research. Molecular Brain Research·P Peinado-RamónF Hallböök
Sep 30, 1998·Proceedings of the National Academy of Sciences of the United States of America·B MurrayE J Furshpan

❮ Previous
Next ❯

Citations

May 20, 2015·Neurochemistry International·Mark William LopesRodrigo Bainy Leal
Oct 13, 2009·Seizure : the Journal of the British Epilepsy Association·Zu-Cai XuKe-Bin Zeng
Feb 5, 2010·Environmental Management·Yongyut TrisuratPeter H Verburg
Jul 27, 2004·Science·Christophe BernardDaniel Johnston

❮ Previous
Next ❯

Related Concepts

Related Feeds

Amygdala: Sensory Processes

Amygdalae, nuclei clusters located in the temporal lobe of the brain, play a role in memory, emotional responses, and decision-making. Here is the latest research on sensory processes in the amygdala.