Phosphorylation of paxillin tyrosines 31 and 118 controls polarization and motility of lymphoid cells and is PMA-sensitive

Journal of Cell Science
Larisa Y RomanovaJ Frederic Mushinski

Abstract

Tyrosine phosphorylation of paxillin regulates actin cytoskeleton-dependent changes in cell morphology and motility in adherent cells. In this report we investigated the involvement of paxillin tyrosine phosphorylation in the regulation of actin cytoskeleton-dependent polarization and motility of a non-adherent IL-3-dependent murine pre-B lymphocytic cell line Baf3. We also assessed the effect of phorbol myristate acetate (PMA), a phorbol ester analogous to those currently in clinical trials for the treatment of leukemia, on paxillin phosphorylation. Using tyrosine-to-phenylalanine phosphorylation mutants of paxillin and phosphospecific antibody we demonstrated that IL-3 stimulated phosphorylation of paxillin tyrosine residues 31 and 118, whereas the tyrosines 40 and 181 were constitutively phosphorylated. Phosphorylation of paxillin residues 31 and 118 was required for cell polarization and motility. In the presence of IL-3, PMA dramatically reduced the phosphorylation of residues 31 and 118, which was accompanied by inhibition of cell polarization and motility. This PMA effect was partially recapitulated by expression of exogenous tyrosine 31 and 118 mutants of paxillin. We also demonstrated that PMA inhibited the IL-3-induce...Continue Reading

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Citations

Dec 8, 2005·Breast Cancer Research and Treatment·Stephen HiscoxRobert I Nicholson
Apr 15, 2010·Journal of Cell Science·Larisa Y RomanovaJ Frederic Mushinski
Jul 6, 2010·Experimental Cell Research·Soon-Mi LimAndreea Trache
Mar 31, 2015·Journal of Cellular Physiology·Huihui WangHuanxiang Zhang
Jun 12, 2014·American Journal of Respiratory Cell and Molecular Biology·Ina HermannJan-Peter Hildebrandt
Mar 17, 2005·World Journal of Gastroenterology : WJG·Hai-Gang LiYun-Jie Zeng
Jul 21, 2007·The Journal of Biological Chemistry·Valerie Chew, Kong-Peng Lam
Nov 22, 2018·PloS One·Yuhsuke OhmiKeiko Furukawa
Mar 22, 2015·American Journal of Physiology. Lung Cellular and Molecular Physiology·Panfeng FuViswanathan Natarajan
Jan 4, 2019·Genes & Genomics·Ae Sun Nah, Kee Oh Chay

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