Phosphorylation of plasma membrane aquaporin PIP2;1 in C-terminal affects light-induced stomatal opening in Arabidopsis.

Plant Signaling & Behavior
Cai-Jiao HuangYu-Ling Chen

Abstract

Guard cells undergo quick volume changes during stomatal movements. However, the contribution of aquaporins to stomatal movements has not been well understood. The plasma membrane aquaporin PIP2;1in Arabidopsis has been found to mediate abscisic acid-induced or flag22-induced stomatal closure. In this research, we investigated the role of PIP2;1 in light-induced stomatal opening by measuring the stomatal apertures of the pip2;1 mutant and PIP2;1 overexpression lines after light treatment. pip2;1 mutant exhibited a larger stomatal aperture, and the overexpression lines displayed a smaller stomatal aperture. It has been reported that the phosphorylation at Ser-280 and Ser-283 of PIP2;1 in rosette tissue increased in response to darkness, whereas osmotic water permeability (P f) in mesophyll protoplasts in darkness was lower than that under light, suggesting that phosphorylation at Ser-280 and Ser-283 of PIP2;1 affected P f in mesophyll protoplasts. Therefore, we obtained the pip2;1 mutant expressing phosphorylation-deficient (PIP2;1 AA) or phosphomimetic (PIP2;1 DD) forms of PIP2;1. The PIP2;1 AA lines exhibited a larger stomatal aperture as pip2;1 mutant, whereas PIP2;1 DD lines exhibited a smaller stomatal aperture as PIP2;1 ov...Continue Reading

References

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May 4, 2017·Plant Physiology·Shin-Ichiro Inoue, Toshinori Kinoshita
Aug 9, 2017·Proceedings of the National Academy of Sciences of the United States of America·Olivier RodriguesLionel Verdoucq
Dec 25, 2019·Journal of Experimental Botany·Jack S A MatthewsTracy Lawson

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Methods Mentioned

BETA
transgenic
Polymerase Chain Reaction

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