Phosphorylation of protein-tyrosine phosphatase PTP-1B on identical sites suggests activation of a common signaling pathway during mitosis and stress response in mammalian cells.

The Journal of Biological Chemistry
V ShifrinB G Neel

Abstract

PTP-1B is a widely expressed non-transmembrane tyrosine-specific phosphatase. Previous studies indicated that, at mitosis, PTP-1B undergoes phosphorylation on two sites, 352Ser-Pro-Leu-Asn and 386Ser-Pro-Ala-Lys. Although the Ser-386 site can be phosphorylated by Cyclin B/Cdc2 in vitro, the kinase for the Ser-352 site is unknown. We have found that these phosphorylation events are not unique to normal mitosis. Instead, treatment with many, but not all, stress stimuli, in particular osmotic shock and certain phosphatase and protein synthesis inhibitors, leads to phosphorylation of PTP-1B. Tryptic phosphopeptide and mutant analysis reveals that, as in mitosis, stress-induced PTP-1B phosphorylation involves both Ser-352 and Ser-386. Activation of the proline-directed kinases Erk1/2, JNKs, and p38 was neither necessary nor sufficient for stress-induced PTP-1B phosphorylation. Our data suggest the existence of a novel mitogen-activated protein kinase pathway in mammalian cells, which is activated at mitosis and in response to osmotic shock and other stresses and results in PTP-1B phosphorylation. This pathway may be similar to the recently described Spc1/Sty1 pathway in Schizosaccharomyces pombe.

References

May 18, 1990·Cell·S Moreno, P Nurse
Jan 23, 1995·Gene·M A Sells, J Chernoff
Aug 15, 1995·Proceedings of the National Academy of Sciences of the United States of America·D T DudleyA R Saltiel
Mar 19, 1993·Science·J L BrewsterM C Gustin
May 26, 1995·The Journal of Biological Chemistry·S MatsudaE Nishida
Oct 11, 1994·Proceedings of the National Academy of Sciences of the United States of America·G HannigR L Erikson
Aug 5, 1994·Science·Z Galcheva-GargovaR J Davis
May 12, 1994·Nature·J M KyriakisJ R Woodgett
May 1, 1993·Molecular Biology of the Cell·G L SchievenJ A Ledbetter

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Citations

Mar 15, 2001·Current Opinion in Cell Biology·N K Tonks, B G Neel
Apr 1, 1997·Current Opinion in Cell Biology·B G Neel, N K Tonks
Apr 8, 1998·Current Opinion in Genetics & Development·D Van VactorB G Neel
Apr 13, 2010·Trends in Biochemical Sciences·Shu-Chin YipJonathan Chernoff
Sep 30, 2014·Seminars in Cell & Developmental Biology·Jesse Bakke, Fawaz G Haj
Mar 28, 1998·The Journal of Biological Chemistry·X ZhaoK Palczewski
Dec 1, 2006·The Journal of Biological Chemistry·Kristen M PichaLawrence P Wennogle
Mar 13, 2012·Pharmacological Reviews·E J Verspohl
Sep 10, 1999·The Journal of Biological Chemistry·F M MoesleinG E Landreth
Nov 9, 2002·The Journal of Biological Chemistry·Fawaz G HajBenjamin G Neel
Feb 16, 2002·The Journal of Biological Chemistry·Kamala P KesavanMarietta L Harrison
May 6, 2008·The Journal of Biological Chemistry·Yupeng ChenYongfeng Shang
Apr 16, 2021·The Journal of Investigative Dermatology·Hyun-Su LeeChang-Duk Jun
Jul 1, 2001·The New Phytologist·Sheng LuanRajeev Gupta

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