Abstract
Phosphatase and tensin homolog deleted on chromosome Ten (PTEN), a tumor suppressor protein participates in multiple cellular activities including DNA repair. In this work we found a relationship between phosphorylation of carboxy (C)-terminal STT motif of PTEN and DNA damage response. Ectopic expression of C-terminal phospho-mutants of PTEN, in PTEN deficient human glioblastoma cells, U87MG, resulted in reduced viability and DNA repair after etoposide induced DNA damage compared to cells expressing wild type PTEN. Also, after etoposide treatment phosphorylation of PTEN increased at C-terminal serine 380 and threonine 382/383 residues in PTEN positive HEK293T cells and wild type PTEN transfected U87MG cells. One-step further, DNA damage induced phosphorylation of PTEN was confirmed by immunoprecipitation of total PTEN from cellular extract followed by immunobloting with phospho-specific PTEN antibodies. Additionally, phospho-PTEN translocated to nucleus after etoposide treatment as revealed by indirect immunolabeling. Further, phosphorylation dependent nuclear foci formation of PTEN was observed after ionizing radiation or etoposide treatment which colocalized with γH2AX. Additionally, etoposide induced γH2AX, Mre11 and Ku70 fo...Continue Reading
References
Mar 1, 1988·Experimental Cell Research·N P SinghE L Schneider
Oct 20, 1998·Cell·V StambolicT W Mak
May 26, 1999·Proceedings of the National Academy of Sciences of the United States of America·H SunH Wu
Sep 1, 1999·Proceedings of the National Academy of Sciences of the United States of America·M M GeorgescuH Hanafusa
Jun 24, 2000·Molecular and Cellular Biology·F VazquezW R Sellers
Nov 15, 2001·The Journal of Biological Chemistry·F VazquezW R Sellers
Mar 23, 2004·Biomedical Papers of the Medical Faculty of the University Palacký, Olomouc, Czechoslovakia·Alice HlobilkováZdenek Kolár
Jun 15, 2004·The Biochemical Journal·Nick R Leslie, C Peter Downes
May 19, 2005·Cancer Research·Ji-Hyun ChungCharis Eng
Jul 1, 2005·Molecular and Cellular Biology·Juinn-Lin LiuW K Alfred Yung
Mar 16, 2006·Proceedings of the National Academy of Sciences of the United States of America·Francisca VazquezPeter N Devreotes
Jan 16, 2007·Cell·Wen Hong ShenYuxin Yin
Apr 14, 2007·Cancer Gene Therapy·G PappasR E Meyn
May 22, 2007·The Journal of Biological Chemistry·Gary D KaoAmit Maity
Jun 27, 2008·Human Molecular Genetics·Glenn P LoboCharis Eng
Jun 12, 2009·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Md Kamruzzaman PramanikShigehiko Yumura
Aug 19, 2010·Protein Science : a Publication of the Protein Society·Roberta E RedfernAlonzo H Ross
Feb 9, 2011·Molecular and Cellular Biology·Jessica A NealKatheryn Meek
Mar 10, 2011·Cancer Research·Tingting GuYuxin Yin
May 31, 2011·Cell Cycle·Michael FraserRobert G Bristow
Jul 21, 2011·Cancer Research·Mei MingYu-Ying He
Apr 5, 2012·Nature Reviews. Molecular Cell Biology·Min Sup SongPier Paolo Pandolfi
Jun 26, 2013·Mutation Research·Ananda MukherjeeParimal Karmakar
Sep 21, 2013·Cell Cycle·Byeong Hyeok ChoiWei Dai
Feb 25, 2014·Cell Reports·Zhuo SunYuxin Yin
Citations
May 4, 2016·PloS One·Miriam StumpfJeroen den Hertog
Nov 8, 2017·Cell Cycle·Prerna MalaneyVrushank Davé
Apr 19, 2017·Oncotarget·Christoph BurdelskiWaldemar Wilczak
May 16, 2019·DNA Repair·Deblina GhoshParimal Karmakar
Feb 13, 2021·Cancer Research·Evan K DayMatthew J Lazzara
Aug 7, 2021·DNA Repair·Sandip MisraParimal Karmakar