Phosphorylation of Rab11-FIP2 regulates polarity in MDCK cells.

Molecular Biology of the Cell
Lynne A LapierreJames R Goldenring

Abstract

The Rab11 effector Rab11-family interacting protein 2 (Rab11-FIP2) regulates transcytosis through its interactions with Rab11a and myosin Vb. Previous studies implicated Rab11-FIP2 in the establishment of polarity in Madin-Darby canine kidney (MDCK) cells through phosphorylation of Ser-227 by MARK2. Here we examine the dynamic role of Rab11-FIP2 phosphorylation on MDCK cell polarity. Endogenous Rab11-FIP2 phosphorylated on Ser-227 coalesces on vesicular plaques during the reestablishment of polarity after either monolayer wounding or calcium switch. Whereas expression of the nonphosphorylatable Rab11-FIP2(S227A) elicits a loss in lumen formation in MDCK cell cysts grown in Matrigel, the putative pseudophosphorylated Rab11-FIP2(S227E) mutant induces the formation of cysts with multiple lumens. On permeable filters, Rab11-FIP2(S227E)-expressing cells exhibit alterations in the composition of both the adherens and tight junctions. At the adherens junction, p120 catenin and K-cadherin are retained, whereas the majority of the E-cadherin is lost. Although ZO-1 is retained at the tight junction, occludin is lost and the claudin composition is altered. Of interest, the effects of Rab11-FIP2 on cellular polarity did not involve myosin ...Continue Reading

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Citations

Jan 25, 2013·Molecular Biology of the Cell·Moorthy KrishnanJames R Goldenring
Apr 4, 2014·Molecular Biology of the Cell·Ruifeng LuJean M Wilson
Jun 4, 2014·The Journal of Clinical Investigation·Byron C KnowlesMitchell D Shub
Jan 23, 2016·Experimental Cell Research·Jenny C SchaferJames R Goldenring
Mar 29, 2016·Cellular and Molecular Gastroenterology and Hepatology·Victoria G WeisJames R Goldenring
Sep 24, 2013·Biochimica Et Biophysica Acta·Patrick LallAmir R Khan
May 30, 2015·Current Opinion in Cell Biology·James R Goldenring
May 26, 2015·Nature Neuroscience·Zhen ZhaoBerislav V Zlokovic
Feb 24, 2017·Molecular Biology of the Cell·Lynne A LapierreJames R Goldenring
Nov 23, 2016·Molecular and Cellular Biology·Carl LaflammeGregory Emery
Jul 23, 2013·American Journal of Physiology. Gastrointestinal and Liver Physiology·Scott W MessengerGuy E Groblewski
Apr 12, 2017·Cellular Logistics·Rebecca McRaeJames R Goldenring
Mar 29, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Arnab DattaMark A Knepper
Feb 19, 2017·Cold Spring Harbor Perspectives in Biology·Maria Daniela Garcia-CastilloWayne I Lencer
Mar 5, 2016·Cancer Research·Pierre-Luc BoulayWilliam J Muller
Jun 1, 2021·The Journal of Biological Chemistry·Sayantani GoswamiNan Gao

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Methods Mentioned

BETA
GTPase
two-hybrid
PCR
GTPases

Software Mentioned

FV1000
Image J
Photoshop
ImageJ

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