PMID: 2492665Feb 1, 1989Paper

Phosphorylation of RAS1 and RAS2 proteins in Saccharomyces cerevisiae

Proceedings of the National Academy of Sciences of the United States of America
A R CobitzF Tamanoi

Abstract

RAS1 and RAS2 proteins of Saccharomyces cerevisiae are guanine nucleotide-binding proteins involved in the regulation of adenylate cyclase. In this paper, we report that these proteins are phosphorylated. The phosphorylation of RAS1 protein is demonstrated by treating with alkaline phosphatase as well as by labeling with [32P]orthophosphate. The phosphorylation occurs exclusively on serine residues and phosphorylated RAS1 protein is predominantly membrane localized. The phosphorylation of RAS2 protein is demonstrated by similar 32P-labeling experiments. The phosphorylation occurs exclusively on serine residues and phosphopeptide analyses suggest that only two major phosphorylated tryptic peptides are generated from the RAS2 protein. These results provide evidence for the phosphorylation of RAS proteins in vivo. Furthermore, our demonstration that the phosphorylation occurs exclusively on serine residues and that the RAS2 protein contains only two major phosphorylated tryptic peptides argues that the phosphorylation may be physiologically significant.

References

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Nov 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·K KaibuchiK Matsumoto
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Citations

May 25, 1993·Biochimica Et Biophysica Acta·C M Newman, A I Magee
Jan 1, 1997·Cellular Signalling·S ArimuraY Watanabe
Jul 23, 2011·Genes & Cancer·Fuyuhiko Tamanoi
Jan 1, 1991·Advances in Enzyme Regulation·P J RoachT W Hrubey
Apr 24, 2012·Cellular Signalling·Jurgen VandammeJohan M Thevelein
Jul 25, 1997·The Journal of Biological Chemistry·J L Whistler, J Rine
Jun 1, 1989·Microbiological Reviews·J B Gibbs, M S Marshall

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