Phosphorylation of Shc proteins in human sperm in response to capacitation and progesterone treatment

Molecular Reproduction and Development
C MorteP Martínez

Abstract

Several authors have demonstrated the involvement of tyrosine kinases during sperm capacitation and acrosome reaction. Shc proteins (p46Shc, p52Shc, and p66Shc) are cytoplasmic substrates of activated tyrosine kinases and are widely expressed in mammalian somatic tissues. Experiments were designed to demonstrate the presence of Shc in spermatozoa and to study its involvement in the signal transduction events leading to acrosome reaction. Anti-Shc antibodies strongly reacted with the acrosomal region of methanol-fixed human sperm. Only one Shc isoform (p52Shc) was detected on Western blot. To study the degree of phosphorylation of Shc during capacitation and acrosome reaction, sperm samples were divided into two groups: noncapacitated and capacitated/progesterone treated. Lysates from both groups were immunoprecipitated with anti-phosphotyrosine antibodies and the precipitated (i.e., phosphorylated) proteins were tested with anti-Shc antibodies. The intensity of p52Shc was clearly increased in capacitated/progesterone-stimulated cells.

References

Feb 1, 1981·The Journal of Experimental Zoology·P Talbot, R S Chacon
Dec 16, 1994·Science·W M Kavanaugh, L T Williams
Jan 1, 1995·Folia primatologica; international journal of primatology·E R Roldan, M Gomendio

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Citations

Dec 5, 2012·Journal of Signal Transduction·Takashi W IjiriKen-Ichi Sato
Nov 13, 2004·Reproductive Biology and Endocrinology : RB&E·Rajesh K Naz, Preeti B Rajesh
Jan 8, 2008·Molecular and Cellular Endocrinology·Tal Almog, Zvi Naor
Jun 6, 2015·Biochemical and Biophysical Research Communications·Ki Young ChoiSeung Hwan Hong
Oct 19, 2001·Oncogene·K S Ravichandran

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