PMID: 3755998Jul 1, 1986Paper

Phosphorylation of skeletal and cardiac muscle C-proteins by the catalytic subunit of cAMP-dependent protein kinase

Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire
M S Lim, M P Walsh

Abstract

Catecholamines are known to influence the contractility of cardiac and skeletal muscles, presumably via cAMP-dependent phosphorylation of specific proteins. We have investigated the in vitro phosphorylation of myofibrillar proteins by the catalytic subunit of cAMP-dependent protein kinase of fast- and slow-twitch skeletal muscles and cardiac muscle with a view to gaining a better understanding of the biochemical basis of catecholamine effects on striated muscles. Incubation of canine red skeletal myofibrils with the isolated catalytic subunit of cAMP-dependent protein kinase and Mg-[gamma-32P]ATP led to the rapid incorporation of [32P]phosphate into five major protein substrates of subunit molecular weights (MWs) 143,000, 60,000, 42,000, 33,000, and 11,000. The 143,000 MW substrate was identified as C-protein; the 42,000 MW substrate is probably actin; the 33,000 MW substrate was shown not to be a subunit of tropomyosin and, like the 60,000 and 11,000 MW substrates, is an unidentified myofibrillar protein. Isolated canine red skeletal muscle C-protein as phosphorylated to the extent of approximately 0.5 mol Pi/mol C-protein. Rabbit white skeletal muscle and bovine cardiac muscle C-proteins were also phosphorylated by the cataly...Continue Reading

Citations

Sep 6, 2011·Journal of Proteome Research·Maegen A Ackermann, Aikaterini Kontrogianni-Konstantopoulos
Aug 20, 1996·Proceedings of the National Academy of Sciences of the United States of America·A Weisberg, S Winegrad
Apr 17, 2010·Journal of Biomedicine & Biotechnology·Maegen A Ackermann, Aikaterini Kontrogianni-Konstantopoulos
Nov 19, 2013·Pflügers Archiv : European journal of physiology·Vasco SequeiraJolanda van der Velden
Nov 2, 2011·Journal of Molecular Biology·Cy M JeffriesJill Trewhella
Jul 19, 2013·Biochimica Et Biophysica Acta·Vasco SequeiraJolanda van der Velden
Jun 8, 2004·Canadian Journal of Physiology and Pharmacology·S A ReadingJ K Barclay
Jul 22, 1998·Circulation Research·A Weisberg, S Winegrad
May 29, 2004·Circulation Research·Emily FlashmanHugh Watkins
May 18, 1987·Biochimica Et Biophysica Acta·K K SchlenderT J Thysseril
Aug 30, 1988·Biochemical and Biophysical Research Communications·K K SchlenderM G Hegazy

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