PMID: 12782394Jun 5, 2003Paper

Phosphorylation of the alpha4 subunit of human alpha4beta2 nicotinic receptors: role of cAMP-dependent protein kinase (PKA) and protein kinase C (PKC)

Brain Research. Molecular Brain Research
M A PachecoL Wecker

Abstract

This study determined whether the alpha4 subunit of human alpha4beta2 neuronal nicotinic receptors is phosphorylated in situ by cyclic AMP-dependent protein kinase (PKA) or protein kinase C (PKC). To accomplish this, human cloned epithelial cells stably transfected with the human alpha4beta2 nicotinic receptor (SH-EP1-halpha4beta2) were incubated with 32P-orthophosphate to label endogenous ATP stores, and the phosphorylation of alpha4 subunits was determined in the absence or presence of PKA or PKC activation. Autoradiographs and immunoblots indicated that alpha4 subunits immunoprecipitated from a membrane preparation of SH-EP1-halpha4beta2 cells exhibited a single 32P-labeled band corresponding to the alpha4 subunit protein; no signals were associated with untransfected SH-EP1 cells. The alpha4 subunits from SH-EP1-halpha4beta2 cells incubated in the absence of the activators exhibited a basal level of phosphorylation that was decreased in the presence of the PKA inhibitor H-89 (5 microM), but unaltered in the presence of the PKC inhibitor Ro-31-8220 (0.1 microM). Activation of PKA by forskolin (10 microM), dibutyryl-cAMP (1 mM), or Sp-8-Br-cAMP (1 mM) enhanced phosphorylation nearly threefold; the inactive isomer, Rp-8-Br-cAM...Continue Reading

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Citations

Nov 22, 2013·PloS One·Isabel Martinez-Pena y ValenzuelaMohammed Akaaboune
May 30, 2008·Frontiers in Bioscience : a Journal and Virtual Library·Gregg B Wells
Jan 15, 2014·The International Journal of Biochemistry & Cell Biology·Galyna GergalovaMaryna Skok
Jun 8, 2007·Cellular and Molecular Neurobiology·Enrique L M Ochoa, Jose Lasalde-Dominicci
Mar 26, 2009·IUBMB Life·Marios ZouridakisSocrates J Tzartos
Nov 16, 2007·The European Journal of Neuroscience·S LadrechM Lenoir
May 26, 2005·Brain Research. Brain Research Reviews·Hai Wang, Xiulan Sun

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