PMID: 9557662Apr 29, 1998Paper

Phosphorylation of the core protein of hepatitis B virus by a 46-kilodalton serine kinase

Journal of Virology
J H Kau, L.-P. Ting

Abstract

Core protein is the major component of the core particle (nucleocapsid) of human hepatitis B virus. Core particles and core proteins are involved in a number of important functions in the replication cycle of the virus, including RNA packaging, DNA synthesis, and recognition of viral envelope proteins. Core protein is a phosphoprotein with most, if not all, of the phosphorylation on C-terminal serine residues. In this study, we identified a serine kinase activity from the ribosome-associated protein fraction of cytoplasm that could specifically bind and phosphorylate the C-terminal portion of recombinant core protein. This kinase is referred to as core-associated kinase (CAK). CAK could be inhibited by the kinase inhibitors heparin and manganese ions but not by spermidine, DRB, H89, or H7, indicating that CAK is distinct from protein kinase A and protein kinase C. CAK could be partially purified by heparin-Sepharose CL-6B and phosphocellulose P11 columns. By using a far-Western assay, three specific proteins, of 46, 35, and 13 kDa, were shown to interact with the C-terminal part of the core protein. These three proteins were present only in the eluted fractions that contains the CAK activity. An in-gel kinase assay showed that ...Continue Reading

References

Nov 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·S Zhou, D N Standring
Mar 1, 1986·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·M MondelliA Alberti
Apr 1, 1987·Journal of Virology·M J Roossinck, A Siddiqui
Nov 1, 1973·Journal of Virology·P M KaplanW S Robinson
Apr 1, 1980·Journal of Virology·C Albin, W S Robinson
Jan 1, 1993·Archives of Virology. Supplementum·M KannW H Gerlich
Jul 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·C T YehJ H Ou

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Citations

Jun 3, 2006·The Biochemical Journal·Hee Yong KangGuhung Jung
Feb 19, 2013·Expert Review of Vaccines·Kenny RooseXavier Saelens
Oct 14, 2000·Annual Review of Cell and Developmental Biology·G R WhittakerA Helenius
Aug 12, 2003·Proceedings of the National Academy of Sciences of the United States of America·Birgit RabeMichael Kann
Feb 28, 2008·Journal of Cellular Physiology·David H NguyenJianming Hu
Dec 10, 1999·Journal of Virology·M KoschelV Bruss
Sep 12, 2012·Medical Microbiology and Immunology·Reinhild Prange
Feb 23, 2020·Frontiers in Cell and Developmental Biology·Mayuko NishiAkihide Ryo
Nov 18, 2018·Frontiers in Microbiology·Fan Yang
Nov 30, 2004·Virus Research·Volker Bruss
Sep 6, 2011·Retrovirology·Charline GiroudLaurence Briant

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