Phosphorylation of thyroid hormone receptors by protein kinase A regulates DNA recognition by specific inhibition of receptor monomer binding.

The Journal of Biological Chemistry
C Tzagarakis-Foster, M L Privalsky

Abstract

Thyroid hormone receptor (T3R) alpha-1 and its oncogenic derivative, the v-ERB A protein, are phosphorylated by cAMP-dependent protein kinase A. Although this phosphorylation appears to be necessary for the oncogenic properties of v-ERB A, the mechanism by which phosphorylation influences the functions of v-ERB A and of the normal T3R has not been established. The protein kinase A phosphorylation site in T3Ralpha-1 is within a domain that is known to contribute to the DNA recognition properties of these receptors. We therefore analyzed the effects of protein kinase A phosphorylation on DNA recognition by the normal T3Ralpha and by the v-ERB A oncoprotein. We report here that phosphorylation of these receptor derivatives does not significantly alter the overall affinity of receptor dimers for DNA. However, phosphorylation does notably alter DNA recognition by preventing, or greatly inhibiting, the ability of these receptors to bind to DNA as protein monomers. These studies suggest that the phosphorylation of T3Ralpha-1 and v-ERB A by protein kinase A may provide a means of altering promoter recognition through a post-translational modification.

References

Sep 14, 1992·Biochimica Et Biophysica Acta·M L Privalsky
Feb 1, 1992·Endocrine Reviews·E OrtíA Munck
Aug 7, 1992·Cell·T Hunter, M Karin
Dec 11, 1990·Biochimica Et Biophysica Acta·C K Glass, J M Holloway
Dec 18, 1986·Nature·C WeinbergerR M Evans
Jun 30, 1995·Annals of the New York Academy of Sciences·R C RibeiroJ D Baxter
May 1, 1995·Molecular and Cellular Biology·D KatzM A Lazar
Feb 1, 1995·Current Biology : CB·V Laudet, G Adelmant
Jan 17, 1995·Proceedings of the National Academy of Sciences of the United States of America·K A OlsonB L Vallee
Jan 1, 1994·Thyroid : Official Journal of the American Thyroid Association·W W Chin
Jan 1, 1994·Annual Review of Biochemistry·M J Tsai, B W O'Malley
Apr 1, 1994·Molecular and Cellular Endocrinology·G G Kuiper, A O Brinkmann
Aug 16, 1994·Proceedings of the National Academy of Sciences of the United States of America·M K BhatS Y Cheng
Feb 1, 1993·Journal of Virology·S SandeM Privalsky
Dec 15, 1995·Cell·D J MangelsdorfR M Evans
Sep 6, 1996·The Journal of Biological Chemistry·D C LeitmanR C Ribeiro
Jul 1, 1996·Chemistry & Biology·J A Katzenellenbogen, B S Katzenellenbogen
Nov 1, 1996·The Biochemical Journal·N L Weigel
Apr 11, 1997·The Journal of Biological Chemistry·B C LinM L Privalsky

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Citations

Dec 2, 1999·Biochimica Et Biophysica Acta·F I Comer, G W Hart
Jul 12, 2005·Oncogene·Sangho Lee, Martin L Privalsky
Oct 4, 2007·Thyroid : Official Journal of the American Thyroid Association·Olga TurowskaMonika Puzianowska-Kuznicka
Feb 19, 2008·Thyroid : Official Journal of the American Thyroid Association·J Enrique Silva, Suzy D C Bianco
Jun 9, 2000·Annual Review of Physiology·J Zhang, M A Lazar
Dec 24, 2013·Journal of Molecular Endocrinology·Raffaella Maria Gadaleta, Luca Magnani
Jun 22, 1999·The Journal of Clinical Investigation·D Shao, M A Lazar
Nov 4, 2015·Molecular and Cellular Endocrinology·Vincent R RoggeroLizabeth A Allison
Feb 7, 2007·Developmental Dynamics : an Official Publication of the American Association of Anatomists·L JiC C Helbing
Nov 28, 2008·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Rachel C SkirrowCaren C Helbing
Jun 20, 2007·Biochemical and Biophysical Research Communications·Kazuki MochizukiToshinao Goda
Jun 17, 2006·Molecular and Cellular Endocrinology·E R Nelson, H R Habibi
Apr 8, 2006·Physiological Reviews·J Enrique Silva
Jan 15, 2005·Molecular Endocrinology·Ghislain M C BonamyLizabeth A Allison
Jun 28, 2001·Physiological Reviews·P M Yen
Jun 26, 2020·Physiological Reviews·Paul J DavisHung-Yun Lin
Aug 2, 2003·Molecular and Cellular Endocrinology·James B NicollLizabeth A Allison
Sep 22, 2021·Proceedings of the National Academy of Sciences of the United States of America·Svetlana MinakhinaFredric E Wondisford
Dec 26, 2001·Chemical Reviews·J B Shabb

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