PMID: 9178492May 1, 1997Paper

Phosphorylation of tick-borne encephalitis virus NS5 protein

Virus Research
O V Morozova Kit YYa

Abstract

The largest tick-borne encephalitis virus (TBEV) non-structural protein NS5 (100 kDa) is believed to be involved in RNA replication. The protein is phosphorylated in infected cell extracts in the presence of [gamma-32P]ATP, as shown by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blot analysis using monoclonal antibodies raised against TBEV NS5 protein. Radioactive labeling of NS5 in cellular extracts at an early stage post-infection is higher than at 24 h post-infection. Incubation of immunoprecipitates of NS5 protein with [gamma-32P]ATP in the presence of Mg2+ resulted in the phosphorylation of TBEV NS5 protein and of immunoglobulins. Phosphoamino acid analysis demonstrated that NS5 contains phosphoserine, but not phosphothreonine, or phosphotyrosine.

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Citations

Dec 22, 1999·Journal of Viral Hepatitis·A Bartholomeusz, P Thompson
Jul 10, 2009·Journal of Virology·Dipankar BhattacharyaRob Striker
Jul 24, 2013·Virology Journal·Julie A KeatingRob Striker
Feb 3, 2009·Virus Research·Dipankar BhattacharyaRob Striker
Oct 16, 2007·Biochimica Et Biophysica Acta·Gualtiero AlvisiDavid A Jans
Nov 25, 2011·Reviews in Medical Virology·Julie A Keating, Rob Striker
Sep 2, 2008·Virology·Dipankar BhattacharyaRob Striker
Jan 20, 2016·Expert Reviews in Molecular Medicine·Mahmoud IranpourSaeid Ghavami
Dec 19, 2018·Viruses·Stephanie ThurmondRong Hai
Aug 11, 2021·Viruses·Thanyaporn DechtawewatSansanee Noisakran
Jun 15, 2019·Journal of Proteome Research·Duangnapa KovanichDuncan R Smith
Aug 29, 2021·Viruses·Akatsuki SaitoShige H Yoshimura

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