PMID: 9547259Jun 20, 1998Paper

Phosphorylation of tobacco eukaryotic translation initiation factor 4A upon pollen tube germination

Nucleic Acids Research
R G op den Camp, C Kuhlemeier

Abstract

Eukaryotic translation initiation factor eIF-4A is a member of the DEAD box family of RNA helicases and RNA-dependent ATPases. In tobacco, eIF-4A is encoded by a gene family with one isoform, eIF-4A8, being exclusively expressed in pollen. This pollen-specific isoform is a candidate for mediating translational control in the developing gametophyte. Here we show that eIF-4A is barely phosphorylated in mature pollen, but during pollen tube germination, two isoforms of eIF-4A become phosphorylated. Phosphoamino acid analysis indicated phosphorylation of threonine. In order to determine whether pollen-specific eIF-4A8 is among the phosphorylated isoforms, we raised transgenic tobacco plants overexpressing eIF-4A8 containing a histidine tag. Hereby, we could show that indeed eIF-4A8 is modified through phosphorylation. The biological relevance of the phosphorylation of eIF-4A is discussed.

References

Aug 1, 1987·Archives of Biochemistry and Biophysics·R F Duncan, J W Hershey
Dec 1, 1994·Plant Molecular Biology·G W OwttrimC Kuhlemeier
Oct 1, 1993·The Plant Cell·R B GoldbergP M Sanders
Mar 15, 1996·European Journal of Biochemistry·V M Pain
Oct 1, 1996·The Plant Journal : for Cell and Molecular Biology·N BateD Twell

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Citations

May 14, 1999·Trends in Plant Science·J Bailey-Serres
Jun 23, 2006·Nucleic Acids Research·George W Owttrim
Jun 1, 2005·Mechanisms of Development·Greco Hernández, Paula Vazquez-Pianzola
Aug 21, 2007·Molecular & Cellular Proteomics : MCP·Oliver ValeriusGerhard H Braus
Oct 25, 2001·The Journal of Biological Chemistry·Heike Uhlmann-SchifflerHans Stahl
Nov 23, 2007·Biochemical Society Transactions·O A PierratJ H Doonan

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