Phosphorylation of VACM-1/Cul5 by protein kinase A regulates its neddylation and antiproliferative effect.

The Journal of Biological Chemistry
Shirley E BradleyMaria A Burnatowska-Hledin

Abstract

Expression of the VACM-1/cul5 gene in endothelial and in cancer cell lines in vitro inhibits cellular proliferation and decreases phosphorylation of MAPK. Structure-function analysis of the VACM-1 protein sequence identified consensus sites specific for phosphorylation by protein kinases A and C (PKA and PKC) and a Nedd8 protein modification site. Mutations at the PKA-specific site in VACM-1/Cul5 ((S730A)VACM-1) sequence resulted in increased cellular growth and the appearance of a Nedd8-modified VACM-1/Cul5. The aim of this study was to examine if PKA-dependent phosphorylation of VACM-1/Cul5 controls its neddylation status, phosphorylation by PKC, and ultimately growth. Our results indicate that in vitro transfection of rat adrenal medullary endothelial cells with anti-VACM-1-specific small interfering RNA oligonucleotides decreases endogenous VACM-1 protein concentration and increases cell growth. Western blot analysis of cell lysates immunoprecipitated with an antibody directed against a PKA-specific phosphorylation site and probed with anti-VACM-1-specific antibody showed that PKA-dependent phosphorylation of VACM-1 protein was decreased in cells transfected with (S730A)VACM-1 cDNA when compared with the cytomegalovirus-tra...Continue Reading

References

Jun 1, 1995·The American Journal of Physiology·M A Burnatowska-HledinD L Dewitt
Nov 14, 1997·The Journal of Biological Chemistry·T KamitaniE T Yeh
Feb 10, 1999·The American Journal of Physiology·M Burnatowska-HledinB Card
Mar 27, 1999·Biochemical and Biophysical Research Communications·H WadaT Kamitani
May 13, 1999·Proceedings of the National Academy of Sciences of the United States of America·D LiakopoulosA Pause
Jul 18, 2000·American Journal of Physiology. Cell Physiology·M Burnatowska-HledinL Listenberger
Jun 21, 2001·Endothelium : Journal of Endothelial Cell Research· Burnatowska-HledinC Barney
Apr 2, 2003·Nature Reviews. Drug Discovery·Janet Dancey, Edward A Sausville
Aug 15, 2003·American Journal of Physiology. Cell Physiology·C Van DortM Burnatowska-Hledin
Sep 5, 2003·The Journal of Biological Chemistry·Elizabeth C AlappatMarcus E Peter
Dec 23, 2003·Cancer Biology & Therapy·Yi Sun
Jan 21, 2004·Cardiovascular Research·Joerg HerrmannAmir Lerman
Mar 17, 2004·Oncogene·Zhen-Qiang PanKenneth Wu
May 13, 2004·Journal of Cardiovascular Magnetic Resonance : Official Journal of the Society for Cardiovascular Magnetic Resonance·Gerald M Pohost
Jun 9, 2004·Biochemical and Biophysical Research Communications·Maria A Burnatowska-HledinChristopher C Barney
Feb 3, 2005·Nature Reviews. Molecular Cell Biology·Matthew D Petroski, Raymond J Deshaies
May 11, 2005·Traffic·Alessandra d'AzzoTommaso Nastasi
Feb 1, 2006·Proceedings of the National Academy of Sciences of the United States of America·Ewen GallagherMichael Karin
Sep 1, 2006·The Journal of Biological Chemistry·Simona DentiElisabetta Bianchi
Oct 26, 2006·The Journal of Biological Chemistry·Gregory D SalinasJohn Marshall
Dec 23, 2006·Molecular and Cellular Biochemistry·Alyssa E JohnsonMaria A Burnatowska-Hledin
Feb 9, 2007·The Journal of Biological Chemistry·Dexing FangZhimin Lu
Mar 14, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Kun LuoXiao-Fang Yu
Apr 7, 2007·The Journal of Biological Chemistry·Koichi NishiyamaHiroki Kurihara
Oct 15, 2008·Molecular Cell·Anjanabha Saha, Raymond J Deshaies
Apr 11, 2009·Nature·Teresa A SoucySteven P Langston

❮ Previous
Next ❯

Citations

Jun 4, 2011·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Steven P LewisMaria A Burnatowska-Hledin
Apr 1, 2016·Cell Division·Fumihiko OkumuraTakumi Kamura
Jun 23, 2015·Tuberculosis·Attinder ChadhaKrishnamurthy Natarajan
Nov 12, 2016·Cell and Tissue Research·Angelica N WillisMaria A Burnatowska-Hledin
Oct 16, 2010·Cell Biology and Toxicology·Justin LubbersMaria A Burnatowska-Hledin
May 11, 2018·PloS One·Bryan KunklerMaria A Burnatowska-Hledin
Mar 2, 2021·Acta Pharmaceutica Sinica. B·Yifan ChenMeidan Ying

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.

Related Papers

APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica
Steven LewisMaria A Burnatowska-Hledin
Cell Biology and Toxicology
Justin LubbersMaria A Burnatowska-Hledin
Biochemical and Biophysical Research Communications
Maria A Burnatowska-HledinChristopher C Barney
© 2022 Meta ULC. All rights reserved