Phosphorylation state of the cap-binding protein eIF4E during viral infection

Virology
M KleijnA A Thomas

Abstract

The eukaryotic translation initiation factor eIF4E, the cap-binding protein, seems to play an essential role in the establishment of the host shut-off after viral infection. Infection with adenovirus and influenza virus caused dephosphorylation of eIF4E and an involvement of a viral protein was suggested. In this report, we studied several other viruses for their ability to change the phosphorylation state of eIF4E, and we looked for the mechanism of eIF4E dephosphorylation. First, it was shown that after encephalomyocarditis virus (EMCV) and poliovirus infection, dephosphorylation of eIF4E occurred. Dephosphorylation of eIF4E was not observed after Semliki Forest virus and reovirus infection. An artificial increase of the level of phosphorylated eIF4E by treating the cells with the phosphatase inhibitor okadaic acid changed neither the kinetics of EMCV and poliovirus infection, nor that of host shut-off. Infections with the uv-treated EMCV showed that the virus binding or entry into the cell initiates eIF4E dephosphorylation. Besides this entry-induced eIF4E dephosphorylation, dephosphorylation was also induced by blocking protein synthesis with the initiation inhibitor pactamycin, or with the elongation inhibitor cycloheximid...Continue Reading

Citations

Apr 5, 2002·Journal of Virology·Ralph FeuerJ Lindsay Whitton
Aug 29, 2009·PLoS Pathogens·Alfredo CastellóYolanda Revilla
May 24, 2014·Nature Reviews. Immunology·Susan CarpenterKatherine A Fitzgerald
Apr 11, 2001·Proceedings of the National Academy of Sciences of the United States of America·W B LottE J Gowans
May 21, 2014·Nature Immunology·Ciriaco A PiccirilloOla Larsson
Jan 10, 2002·Journal of Medical Virology·Jun KatoMasao Omata
Feb 19, 2015·Viruses·Hilda MonteroSilvia I Mora
May 22, 2007·Plant Biotechnology Journal·Byoung-Cheorl KangMolly M Jahn
Dec 3, 2003·The Journal of Biological Chemistry·R Jeremy Nichols, Paula Traktman
Apr 16, 1998·The Journal of Biological Chemistry·M KleijnA A Thomas
Oct 6, 2020·Frontiers in Cellular and Infection Microbiology·Louis-Philippe LerouxMaritza Jaramillo
Sep 28, 2020·FEBS Open Bio·Vicente Morales-GarciaErick de la Cruz-Hernandez
Mar 19, 1999·Trends in Microbiology·R AndinoA V Gamarnik
Apr 27, 1999·The International Journal of Biochemistry & Cell Biology·B Raught, A C Gingras

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.