Phosphorylation status of transcription factor C/EBPalpha determines cell-surface poly-LacNAc branching (I antigen) formation in erythropoiesis and granulopoiesis

Blood
Yuh-Ching TwuLung-Chih Yu

Abstract

The cell-surface straight and branched repeats of N-acetyllactosamine (LacNAc) units, called poly-LacNAc chains, characterize the histo-blood group i and I antigens, respectively. The transition of straight to branched poly-LacNAc chain (i to I) is determined by the I locus, which expresses 3 IGnT transcripts, IGnTA, IGnTB, and IGnTC. Our previous investigation demonstrated that the i-to-I transition in erythroid differentiation is regulated by the transcription factor CCAAT/enhancer binding protein alpha (C/EBPalpha). In the present investigation, the K-562 cell line was used as a model to show that the i-to-I transition is determined by the phosphorylation status of the C/EBPalpha Ser-21 residue, with dephosphorylated C/EBPalpha Ser-21 stimulating the transcription of the IGnTC gene, consequently resulting in I branching. Results from studies using adult erythropoietic and granulopoietic progenitor cells agreed with those derived using the K-562 cell model, with lentiviral expression of C/EBPalpha in CD34(+) hematopoietic cells demonstrating that the dephosphorylated form of C/EBPalpha Ser-21 induced the expression of I antigen, granulocytic CD15, and also erythroid CD71 antigens. Taken together, these results demonstrate tha...Continue Reading

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Citations

Apr 5, 2012·Blood·Rositsa I KolevaJarrod A Marto
Mar 12, 2013·Biochemical and Biophysical Research Communications·Zhao-Xia WuLi-Shun Wang
May 4, 2013·Molecular and Cellular Endocrinology·Juan RenShemin Lu
Jun 30, 2018·European Journal of Haematology·Vered YahalomElon Pras
Jul 23, 2019·FEBS Letters·Prathyush PothukuchiSeetharaman Parashuraman
Mar 5, 2021·The British Journal of Dermatology·M PerezC J Dimitroff

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