Phosphothreonine 218 is required for the function of SR45.1 in regulating flower petal development in Arabidopsis

Plant Signaling & Behavior
Xiao-Ning ZhangBret Cooper

Abstract

RNA splicing is crucial to the production of mature mRNAs (mRNA). In Arabidopsis thaliana, the protein Arginine/Serine-rich 45 (SR45) acts as an RNA splicing activator and initiates the spliceosome assembly. SR45 is alternatively spliced into 2 isoforms. Isoform 1 (SR45.1) plays an important role in the flower petal development whereas isoform 2 (SR45.2) is important for root growth. In this study, we used immunoprecipitation to isolate an SR45.1-GFP fusion protein from transgenic plants complementing a null mutant, sr45-1. Mass spectrometry suggested a single phosphorylation event in a peptide from the alternatively spliced region unique to SR45.1. Substituting alanine for threonine 218, a candidate site for phosphorylation, did not complement the sr45-1 mutant with narrow flower petals whereas substituting aspartic acid or glutamic acid for threonine 218 did complement the sr45-1 mutant. Mass spectrometry also revealed that other proteins involved in the spliceosome co-precipitated with SR45.1, and RT-qPCR revealed that phosphorylation of threonine 218 promotes the function of SR45.1 in promoting the constitutive splicing of SR30 mRNA. This is the first demonstration of a specific phosphorylation site that differentially regu...Continue Reading

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Citations

Jun 21, 2015·BMC Bioinformatics·Padmini SrinivasanCaren Chang
Feb 24, 2017·International Journal of Molecular Sciences·Xudong ShangLigeng Ma
Jan 31, 2020·Molecular Plant-microbe Interactions : MPMI·Bret CooperKimberly B Campbell
Nov 20, 2016·Plant, Cell & Environment·Albrecht G von Arnim, Hanjo A Hellmann
Dec 25, 2015·Plant Physiology·Nancy StankovicPatrick Motte

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