Phosphotyrosine antibodies specifically label ameboid microglia in vitro and ramified microglia in vivo.

Glia
M L Tillotson, J G Wood

Abstract

Using an affinity-purified, polyclonal antibody to phosphotyrosine (Wang: Molecular and Cellular Biology 5:3640-3643, 1985) we have previously demonstrated that phosphotyrosine immunoreactivity is restricted to a population of multipolar GFAP-negative neuroglia in adult rat brain (Tillotson and Wood: Journal of Comparative Neurology 282:133-141, 1989) and retina (Tillotson and Wood: Journal of Cell Biology 107:724a, 1988). In this study, we show that the phosphotyrosine-immunoreactive cells are microglia. This conclusion is supported by numerous morphological and ultrastructural similarities between the phosphotyrosine-immunoreactive cells and microglia. Furthermore, phosphotyrosine co-localizes with the microglial-specific B4 isolectin of Bandeiraea simplifolia-1 lectin. Phosphotyrosine antibodies also stain ameboid microglia in primary cultures of neonatal rat brain. In addition, after 7 days in vitro, microglia are the only phosphotyrosine-immunoreactive element in the cultures. This temporal pattern of staining in vitro mimics the developmental progression of phosphotyrosine immunoreactivity in situ, in which a variety of structures stain during postnatal neural development (Tillotson and Wood: Journal of Comparative Neurol...Continue Reading

References

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Citations

Jan 2, 1991·Neuroscience Letters·J G Wood, P Zinsmeister
Jun 25, 2003·Experimental Neurology·Shirley Ann GilmoreJohn D Houlé
Apr 13, 2002·Neuroscience Letters·Anthony P NicholasThomas R Tice
Mar 1, 1993·Current Eye Research·R S Roque, R B Caldwell
Aug 13, 2008·Brain Research·Wiebke WendtChristine C Stichel
Jan 1, 1996·Advances in Neuroimmunology·J Zielasek, H P Hartung
Jun 2, 2006·Brain Research·Anna LeichsenringChristine C Stichel
Jul 26, 2002·Experimental Eye Research·Nisha GuptaAnn H Milam
Sep 1, 1994·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·K KorematsuY Ushio

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