Phosphotyrosine-Mediated Regulation of Enterohemorrhagic Escherichia coli Virulence.

MBio
Colin D RobertsonAnne-Marie Hansen

Abstract

Enteric pathogens with low infectious doses rely on the ability to orchestrate the expression of virulence and metabolism-associated genes in response to environmental cues for successful infection. Accordingly, the human pathogen enterohemorrhagic Escherichia coli (EHEC) employs a complex multifaceted regulatory network to link the expression of type III secretion system (T3SS) components to nutrient availability. While phosphorylation of histidine and aspartate residues on two-component system response regulators is recognized as an integral part of bacterial signaling, the involvement of phosphotyrosine-mediated control is minimally explored in Gram-negative pathogens. Our recent phosphotyrosine profiling study of E. coli identified 342 phosphorylated proteins, indicating that phosphotyrosine modifications in bacteria are more prevalent than previously anticipated. The present study demonstrates that tyrosine phosphorylation of a metabolite-responsive LacI/GalR family regulator, Cra, negatively affects T3SS expression under glycolytic conditions that are typical for the colonic lumen environment where production of the T3SS is unnecessary. Our data suggest that Cra phosphorylation affects T3SS expression by modulating the ex...Continue Reading

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Citations

Jul 3, 2019·Journal of Bacteriology·Landon J GetzNikhil A Thomas
Sep 26, 2019·Cellular and Molecular Life Sciences : CMLS·Manlu Zhu, Xiongfeng Dai
Apr 28, 2020·FEBS Letters·Julie Bonne KøhlerIvan Mijakovic
Dec 31, 2020·Proceedings of the National Academy of Sciences of the United States of America·Tamar SzokeOrna Amster-Choder

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Datasets Mentioned

BETA
GSE103764

Methods Mentioned

BETA
PCR
RNA-Seq
reverse transcription-PCR
electrophoretic mobility shift
electrophoresis
Infrared Imaging

Software Mentioned

PyMol
Circos
Ergatis
AxioVision
EXPRESS
DESeq
Bowtie aligner
MeV
FASTX
HTSeq

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