Photo-activatable Cre recombinase regulates gene expression in vivo

Scientific Reports
Suzanne E SchindlerMarc I Diamond

Abstract

Techniques allowing precise spatial and temporal control of gene expression in the brain are needed. Herein we describe optogenetic approaches using a photo-activatable Cre recombinase (PA-Cre) to stably modify gene expression in the mouse brain. Blue light illumination for 12 hours via optical fibers activated PA-Cre in the hippocampus, a deep brain structure. Two-photon illumination through a thinned skull window for 100 minutes activated PA-Cre within a sub-millimeter region of cortex. Light activation of PA-Cre may allow permanent gene modification with improved spatiotemporal precision compared to standard methods.

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Methods Mentioned

BETA
genetic modification
fluorescence microscopy
PCR
transfection
column chromatography
dot blot
flow cytometry

Software Mentioned

Cre
FlowJo
Visiomorph
PA

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