Photo-biohydrogen Production by Photosensitization with Biologically Precipitated Cadmium Sulfide in Hydrogen-Forming Recombinant Escherichia coli.

Chembiochem : a European Journal of Chemical Biology
Yuki HondaHiroshi Fujii

Abstract

An inorganic-biological hybrid system that integrates features of both stable and efficient semiconductors and selective and efficient enzymes is attractive for facilitating the conversion of solar energy to hydrogen. In this study, we aimed to develop a new photocatalytic hydrogen-production system based on Escherichia coli whole-cell genetically engineered as a biocatalysis for highly active hydrogen formation. The photocatalysis part was obtained by bacterial precipitation of cadmium sulfide (CdS), which is a visible-light-responsive semiconductor. The recombinant E. coli cells were sequentially subjected to CdS precipitation and heterologous [FeFe]-hydrogenase synthesis to yield a CdS@E. coli hybrid capable of light energy conversion and hydrogen formation in a single cell. The CdS@E. coli hybrid achieved photocatalytic hydrogen production with a sacrificial electron donor, thus demonstrating the feasibility of our system and expanding the current knowledge of photosensitization using a whole-cell biocatalyst with a bacterially precipitated semiconductor.

References

Oct 9, 2001·Biotechnology and Bioengineering·C L WangJ D Keasling
Nov 24, 2004·Chemistry & Biology·Rozamond Y SweeneyBrent L Iverson
Jun 3, 2008·Angewandte Chemie·Seung Hyun KangWilfred Chen
Oct 27, 2009·Angewandte Chemie·Sung Ho YangInsung S Choi
Nov 26, 2009·Journal of the American Chemical Society·Erwin ReisnerFraser A Armstrong
Jun 30, 2010·Journal of the American Chemical Society·Katherine A BrownPaul W King
Feb 23, 2012·Journal of the American Chemical Society·Katherine A BrownPaul W King
Feb 26, 2014·Journal of the American Chemical Society·Molly B WilkerGordana Dukovic
Mar 25, 2014·Chemical Reviews·Wolfgang LubitzEdward Reijerse
Jan 28, 2015·Physical Chemistry Chemical Physics : PCCP·James K UtterbackGordana Dukovic
Oct 30, 2016·Proceedings of the National Academy of Sciences of the United States of America·Nikolay KornienkoPeidong Yang
May 23, 2018·Proceedings of the National Academy of Sciences of the United States of America·Yoojin ChoiSang Yup Lee
Oct 3, 2018·Nature Nanotechnology·Hao ZhangPeidong Yang
Nov 18, 2018·Science·Junling GuoNeel S Joshi
Jun 28, 2019·Journal of the American Chemical Society·Yuchen DingPrashant Nagpal

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