Photo-modulation of zinc phthalocyanine-treated breast cancer cell line ZR-75-1 inhibited the normal tumor activity in vitro

Lasers in Medical Science
Arezoo Rezaie Nezhad ZamaniReza Rahbarghazi

Abstract

Regarding post-complication of convenient therapies against breast cancer, the emergence of effective approaches is essential. Photodynamic therapy is touted as a novel invasive therapeutic approach by the application of a photosensitizer promoted by laser irradiation. This study aimed to investigate the combined regime of low-level laser irradiation with zinc phthalocyanine in human breast cancer ZR-75-1 cell line. Cells were treated with 0.01 and 5 μg/ml of ZnPc for 24 h and exposed to radiation (70 mW) for 60 s. Cell viability was evaluated by MTT and flow cytometry. Cell migration capacity was monitored by scratch test, Transwell migration insert, and gelatin zymography. The function of MDR in treated cells was examined by Rhodamine 123 exclusion test. The level of GALNT11 was measured by ELISA. The expression of Bax and Bcl-2 genes was evaluated by real-time PCR. Laser irradiation and zinc phthalocyanine induced cell cytotoxicity in a dose-dependent manner. Flow cytometry analysis showed the induction of apoptotic and necrotic changes in treated cells. We found a reduction in migration rate and MMP-9 activity in cells undergoing the experimental procedure (p < 0.05). Immunofluorescence imaging revealed the intracellular ac...Continue Reading

References

Jul 3, 2003·Journal of the National Cancer Institute·Michael F LeitzmannEdward L Giovannucci
Aug 19, 2007·Biochimica Et Biophysica Acta·Esther BuytaertPatrizia Agostinis
Feb 6, 2008·Lasers in Medical Science·K PlaetzerT Kiesslich
May 24, 2008·International Journal of Oncology·Santiago Rello-VaronaAngeles Villanueva
May 2, 2009·Journal of Photochemistry and Photobiology. B, Biology·C A RobertsonH Abrahamse
Jul 14, 2009·Breast Cancer Research and Treatment·Antoinette HollestelleMieke Schutte
Apr 13, 2010·The International Journal of Biochemistry & Cell Biology·Natalia B Rumie VittarViviana Rivarola
May 28, 2011·CA: a Cancer Journal for Clinicians·Patrizia AgostinisJakub Golab
Sep 3, 2011·Breast Cancer Research : BCR·Deborah L Holliday, Valerie Speirs
Mar 12, 2013·Journal of Controlled Release : Official Journal of the Controlled Release Society·Alyssa MasterAnirban Sen Gupta
Jan 1, 2011·Cancers·Pawel MrozMichael R Hamblin
Sep 3, 2013·The International Journal of Biochemistry & Cell Biology·Julieta MarinoLeonor P Roguin
Mar 1, 2005·Photodiagnosis and Photodynamic Therapy·Ana P CastanoMichael R Hamblin
Dec 17, 2014·International Journal of Molecular Sciences·Jorge SorianoMagdalena Cañete
Feb 26, 2015·Cancer Medicine·Kishore R RollakantiEdward V Maytin
Mar 16, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Giovana Maria Fioramonti CalixtoMarlus Chorilli
Jun 21, 2016·Oncotarget·Muhammad Ramzan Manwar HussainMin Fang
May 17, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Sello L ManotoHeidi Abrahamse
Jan 10, 2018·CA: a Cancer Journal for Clinicians·Rebecca L SiegelAhmedin Jemal

❮ Previous
Next ❯

Citations

Feb 23, 2020·Journal of Orthopaedic Surgery and Research·Kai GaoChaoliang Lv
Apr 11, 2020·Journal of Lasers in Medical Sciences·Hesam Saghaei BagheriHojjatollah Nozad Charoudeh
Feb 7, 2021·Nanomedicine : Nanotechnology, Biology, and Medicine·Neeraj S ThakurUttam C Banerjee

❮ Previous
Next ❯

Related Concepts

Related Feeds

BCL-2 Family Proteins

BLC-2 family proteins are a group that share the same homologous BH domain. They play many different roles including pro-survival signals, mitochondria-mediated apoptosis and removal or damaged cells. They are often regulated by phosphorylation, affecting their catalytic activity. Here is the latest research on BCL-2 family proteins.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.